ACT Actin (monoclonal, clone 14H4G8), 10 µg | Agrisera AS21 4615-10 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

ACT Actin (monoclonal, clone 14H4G8), 10 µg

catalog :

AS21 4615-10

quantity :

10 µg

clonality :

recombinant

host :

mouse

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunocytochemistry

product information

Product number :

AS21 4615-10

Product name :

ACT Actin (monoclonal, clone 14H4G8), 10 µg

Immunogen :

ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520

Host :

Mouse

Clonality :

Recombinant

Isotype :

IgG1

Purity :

Immunogen affinity purified serum in PBS pH 7.4.

Format :

Lyophilized

Quantity :

10 µg

Tested applications :

Immunofluorescence (IF), Western blot (WB)

Related products :

AS13 2640 Anti-ACT Actin, rabbit antibodyAS10 681 Anti-tubulin beta chain, rabbit antibodyAS10 680 Anti-tubulin alpha chain, rabbit antibodyAS16 3139 Actin-1, monoclonal antibodyAS16 3140 Actin-2, mouse monoclonal antibodyAS16 3141 Actin-8, mouse monoclonal antibodyAS10 702 Anti-Actin-11, monclonal mouse antibody Plant protein extraction buffer Secondary antibodies

Recommended dilutions :

1: 500 (IF), 1 : 1000 1: 5000 (WB)

Expected apparent MW :

41.6 45 kDa

Confirmed reactivity :

Arabidopsis thaliana, Oryza sativa, Zea mays

Predicted reactivity :

Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao,Triticum aestivum, Zea mays, Vicia fabaSpecies of your interest not listed? Contact us

Not reactive in :

Chlamydomonas reinhardtii

Additional information :

This antibody is compatible with a secondary antibody for fluorescence detection: goat anti-mouse secondary antibody (IRDye 800CW, Li-cor)

Selected references :

To be added when available, antibody released in October 2021.

Background :

Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.

Reconstitution :

For reconstitution add 10 µl, of sterile or deionized water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

UniProt number :

P0CJ46 , Q96292 , P0CJ47 , P53494 , Q8RYC2 , P53492 , Q96293 , P53496 , P53497

TAIR number :

AT2G37620, AT3G18780, AT3G53750, AT5G59370, At2g42100, At5g09810, AT1G49240, AT3G12110, AT3G46520

Research area :

Arabidopsis thaliana