This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Agrisera
product type :
antibody
product name :
Akt/PKB Protein kinase B, (pT308)
catalog :
AS16 4020
quantity :
For 10 Miniblots.
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
human, mouse, rat
application :
western blot
product information
Product number :
AS16 4020
Product name :
Akt/PKB Protein kinase B, (pT308)
Immunogen :
Synthetic phosphopeptide derived from the human VEGFR2 protein which contains Y1054. UniProt: Human: P31749, Mouse: P31750, Rat: P47196
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum
Format :
Liquid
Quantity :
For 10 Miniblots.
Tested applications :
Western blot (WB)
Related products :
anti-rabbit secondary antibodies
Recommended dilutions :
0.35-1.0 µg/mL (WB). At 0.50 µg/mL, the dilution provides 100 mL working solution, which at 10 mL/blot allows 10 blots to be performed. The optimal antibody concentration should be determinedby the end user.
Confirmed reactivity :
Human, Mouse, Rat
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
To be added when available, antibody released in November 2016.
Background :
AKT/Protein Kinase B is a closely related serine/threonine-protein kinases called the AKT kinase. This protein regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt is phosphorylated on thr 308 by PDK1 and on ser 473 by PDK2.
Storage :
Store at -20°C.
Additional information :
Provided in phosphate buffered saline, pH 7.3, with 1.0 mg/ml BSA and 0.05% sodium azide. Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Akt protein. The final product is generated by affinity chromatography using an Akt-derived peptide that is phosphorylated at threonine 308.
UniProt number :
P47196,P31750,P31749
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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