antibody

Anti-H3R2me2(sym)K4me2 Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)

AS16 3185

50 µg

565 USD

polyclonal

domestic rabbit

nonconjugated

western blot, immunocytochemistry, chromatin immunoprecipitation, dot blot

Anti-H3R2me2(sym)K4me2 Histone H3 (sym-dimethylated Arg2, dimethyl Lys4)

Primary antibody

Rabbit

Polyclonal

50 µg

565 USD

Q71DI3

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. The dimethylation of both arginine 2 (H3R2me2) and lysine 4 (H3K4me2) of H3 are both known marks to have opposing affects. R2me2 maintains transcriptional silence by silencing Set1 mediated K4 methylation, in which K4 methylation is normally associated with active chromatin. The protein arginine methyltransferase PRMT6 can methylate H3R2 in vivo, and overexpression of this enzyme downregulates Hox and Myc dependent genes, both of which are targets of H3K4 methylation.

Human

Common histone modifications include methylation of lysine and arginine, acetylation of lysine, phosphorylation of threonine and serine, and sumoylation, biotinylation, and ubiquitylation of lysine. The dimethylation of both arginine 2 (H3R2me2) and lysine 4 (H3K4me2) of H3 are both known marks to have opposing affects. R2me2 maintains transcriptional silence by silencing Set1 mediated K4 methylation, in which K4 methylation is normally associated with active chromatin. The protein arginine methyltransferase PRMT6 can methylate H3R2 in vivo, and overexpression of this enzyme downregulates Hox and Myc dependent genes, both of which are targets of H3K4 methylation.

KLH-conjugated synthetic peptide

Immunogen affinity purified serum.

Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)

Chromatin immunoprecipitation (ChIP), Dot blot (Dot), Immunofluorescence (IF), Western blot (WB)

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.