product summary
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company name :
Agrisera
product type :
antibody
product name :
GFP Green Fluorescence Protein (affinity purified)
catalog :
AS15 2987
quantity :
100 µg at 1 mg/ml
price :
501 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
FITC
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
citations: 3
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product information
Product number :
AS15 2987
Product name :
GFP Green Fluorescence Protein (affinity purified)
Quantity :
100 µg at 1 mg/ml
List Price :
501 USD
Immunogen :
Highly purified native GFP protein derived from Aequorea victoria, UniProt: P42212
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Liquid
Tested applications :
ELISA (ELISA), Immunofluorescence (IF), Immunoprecipitation (IP), Western blot (WB)
Recommended dilutions :
1 : 5000-1 : 25 000 (ELISA), 1: 100 - 1 : 500 (IF), 1 : 2000-1 : 10 000 (WB)
Confirmed reactivity :
Native GFP, Recombinant GFP (E,coli), all variants of GFP, including EGFP
Additional information :
Minimal cross-reactivity with E.coli proteins.Antibody reactivity to mVenus or EBFP2 has not yet been determined.
Selected references :
Sun et al. (2021) The epigenetic factor FVE orchestrates cytoplasmic SGS3-DRB4-DCL4 activities to promote transgene silencing in Arabidopsis. Sci Adv. 2021 Aug 4;7(32):eabf3898. doi: 10.1126/sciadv.abf3898. PMID: 34348894; PMCID: PMC8336953.Stelate et al. (2021) Correlative Light-Environmental Scanning Electron Microscopy of Plasma Membrane Efflux Carriers of Plant Hormone Auxin. Biomolecules. 2021 Sep 26;11(10):1407. doi: 10.3390/biom11101407. PMID: 34680040; PMCID: PMC8533460.Wieczorek et al. (2020) Development of a New Tomato Torrado Virus-Based Vector Tagged with GFP for Monitoring Virus Movement in Plants. Viruses. 2020 Oct 20;12(10):1195. doi: 10.3390/v12101195. PMID: 33092281; PMCID: PMC7588970.Kulich et al. (2018). Deubiquitinase OTU5 affects Root Responses to Phosphate Starvation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693.
Background :
GFP (Green fluorescent protein) was originally identified in photo organs on jellyfish Aequorea victoria. It is a naturally fluorescent protein which emits green light at a maximum wavelength of 509 nm when excited by blue or UV light. It is extensively used in laboratory as a reporter molecule to label and study cellular and subcellular proteins in living cells using a wide range of applications. Antibodies to GFP protein are used in immunoblotting and ELISA. GFP protein has molecular weight of 27 kDa.
Storage :
Store at -20°C. Avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
10 mM TRIS buffer pH 8,0 containing 0,02% sodium azide
UniProt number :
P42212
Commodity Code :
38220000
Our Category :
GFP/EGFP/BFP/CFP/YFP/RFP
Research area :
Tag Antibodies
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.