product summary
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company name :
Agrisera
product type :
antibody
product name :
RBOHD Respiratory burst oxidase homolog protein D
catalog :
AS15 2962
quantity :
50 µg
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunocytochemistry
more info or order :
citations: 1
product information
Product number :
AS15 2962
Product name :
RBOHD Respiratory burst oxidase homolog protein D
Quantity :
50 µg
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated peptide chosen from Arabidopsis thaliana RBOHD sequence, UniProt:Q9FIJ0, TAIR: AT5G47910
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
Immunofluorescence (IF), Western blot (WB)
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
104 kDa
Confirmed reactivity :
Arabidopsis thaliana, Solanum sp.
Predicted reactivity :
Brassica napus, Citrus sinensis, Citrullus colocynthis, Cucumis melo, Fragaria ananassa, Gossypium arboreum, Manihot esculenta, Morus notabilis, Nicotiana tabacum, Oryza sativa, Picea abies, Ricinus communis, Sinensis osbeck, Solanum tuberosum, Theobroma cacao, Zea mays, Zostera marina
Not reactive in :
Marchantia polymorpha
Additional information :
For solubilization we recommend 65°C for 5 min, Sample boiling is not recommended
Related products :
Plant protein extraction buffer Secondary antibodies
Selected references :
Wang et al. (2021) Arabidopsis PUB2 and PUB4 connect signaling components of pattern-triggered immunity. New Phytol. 2021 Dec 17. doi: 10.1111/nph.17922. Epub ahead of print. PMID: 34918346Lee et al. (2020). Regulation of reactive oxygen species during plant immunity through phosphorylation and ubiquitination of RBOHD. Nat Commun. 2020 Apr 15;11(1):1838. doi: 10.1038/s41467-020-15601-5.Jedelská et al. (2019). Tomato Root Growth Inhibition by Salinity and Cadmium Is Mediated By S-Nitrosative Modifications of ROS Metabolic Enzymes Controlled by S-Nitrosoglutathione Reductase. Biomolecules. 2019 Aug 21;9(9). pii: E393. doi: 10.3390/biom9090393.Otulak-Kozie? et al. (2019). The Respiratory Burst Oxidase Homolog D (RbohD) Cell and Tissue Distribution in Potato-Potato Virus Y (PVYNTN) Hypersensitive and Susceptible Reactions. Int. J. Mol. Sci. 2019, 20(11), 2741. (innunofluorescence)
Background :
RBOHD (Respiratory burst oxidase homolog protein D) is a Calcium-dependent NADPH oxidase that generates superoxide. Is involved in the generation of reactive oxygen species (ROS) during incompatible interactions with pathogens and in UV-B and abscisic acid ROS-dependent signaling.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q9FIJ0
TAIR number :
AT5G47910
Commodity Code :
38220000
Our Category :
Plant Oxidative Stress
Research area :
Plant Biology, Enviromental stress
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.