product summary
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company name :
Agrisera
product type :
antibody
product name :
RbcL II Rubisco large subunit, form II
catalog :
AS15 2955
quantity :
50 µl
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot, immunocytochemistry
more info or order :
citations: 1
product information
Product number :
AS15 2955
Product name :
RbcL II Rubisco large subunit, form II
Quantity :
50 µl
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated synthetic peptide conserved in known RbcL form II protein sequences
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Tested applications :
Immunofluorescence (IF), Western blot (WB)
Recommended dilutions :
1:10 000 (IF), (WB)
Expected apparent MW :
51,7 kDa
Confirmed reactivity :
Alexandrium catenella, Amphidinium carterae, Chaetoceros neogracilis, Rhodobacter capsulatus, Rhodospirillum rubrum
Predicted reactivity :
Acidithiobacillus ferrooxidans, Dechloromonas aromatica, Gonyaulax polyedra, Leptothrix cholodnii, Magnetovibrio blakemorei, Rhodobacter sphaeroides, Thiobacillus denitrificans, Rhodopseudomonas palustrisand with a signle mismatch in one amino acid: Gallionella capsiferriformans, Mariprofundus ferrooxydans, Thioalkalicoccus limnaeus, Methanomethylovorans hollandica, Methanococcoides burtonii, Methanosaeta concili, Methanolobus tindarius, Methanohalophilus mahii, and the dinoflagellate Symbiodinium sp. (ex Stylophora pistillata)
Not reactive in :
Burkholderi, Cyanobium sp.
Additional information :
Protein extraction from diatoms protocol can be found here.
Related products :
Plant and algal protein extraction bufferSecondary antibodies
Selected references :
Cho et al. (2021). SxtA localizes to chloroplasts and changes to its 3'UTR may reduce toxin biosynthesis in non-toxic Alexandrium catenella (Group I). Harmful Algae, 2021,101972,ISSN 1568-9883, https://doi.org/10.1016/j.hal.2020.101972. ImmunolocalizationBausch et al. (2019). Combined effects of simulated acidification and hypoxia on the harmful dinoflagellate Amphidinium carterae. Marine Biology, June 2019, 166:80.Long et al. (2018). Carboxysome encapsulation of the CO2-fixing enzyme Rubisco in tobacco chloroplasts. Nat Commun. 2018 Sep 3;9(1):3570. doi: 10.1038/s41467-018-06044-0.
Background :
Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase) catalyzes the rate-limiting step of CO2 fixation in photosynthetic organisms. Form II Rubisco is present in many photosynthetic bacteria and archaea and in some photosynthetic dinoflagellates. The large subunit (LSU) of form I Rubisco is encoded by the RbcL/cbbL gene, while form II LSU is encoded by cbbM.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Additional information :
The antibody does not detect Form I RbcL/cbbL
Commodity Code :
38220000
Our Category :
Global Antibodies
Research area :
Plant Biology, Photosynthesis
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.