product summary
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company name :
Agrisera
product type :
antibody
product name :
TKL1 transketolase (chloroplastic)
catalog :
AS15 2903
quantity :
50 µl
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunoprecipitation
more info or order :
product information
Product number :
AS15 2903
Product name :
TKL1 transketolase (chloroplastic)
Immunogen :
Purified, recombinant mature TKL1 of Arabidopsis thaliana, UniProt: Q8RWV0, TAIR: At3g60750
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Related products :
AS15 2873 Anti-SBP Sedoheptulose-1,7-bis phosphatase, rabbit antibodiescollection of antibodies to carbohydrate metabolismPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 2000 (WB)
Expected apparent MW :
72 kDa
Confirmed reactivity :
Arabidopsis thaliana, Pisum sativum
Predicted reactivity :
Cucumis sativus, Synechococcus elongatus sp PCC 7942 Species of your interest not listed? Contact us
Additional information :
TKL1 has MW of 79.28 kDa with and 73.45 kDa without presequence; about 72 kDa on SDS gelAntibody works on whole leaf extracts and isolated chloroplasts and is also recognizing recombinant TKL protein.This product contains 10% glycerol and might appear as liquid but is provided lyophilized.
Selected references :
Rocha et al. (2014). Phosphorylation of Arabidopsis transketolase at Ser428 provides a potential paradigm for the metabolic control of chloroplast carbon metabolism. Biochem J. 2014 Mar 1;458(2):313-22. doi: 10.1042/BJ20130631.
Background :
TKL-1 (Transketolase-1, chloroplastic) is involved in the pathway of Calvin cycle. This enzyme is catalyzing the reversible transfer of a two-carbon ketol group from fructose-6-phopshate or sedoheptuloze-7-phosphate to glyceraldehyde-3-phosphate to yield xylulose-5-phosphate and erythrose-4-phosphate or ribose-5-phosphate. AtTKL1 is expressed in highest levels in photosynthetic tissue, while AtTKL2 is expressed mainly during embryo development. Alternative name: TK.
Reconstitution :
For reconstitution add 45 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
Q8RWV0
TAIR number :
At3g59780
Research area :
Arabidopsis thaliana
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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