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company name :
Agrisera
product type :
antibody
product name :
Ferritin 1-2 (plant)
catalog :
AS15 2898
quantity :
50 µg
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS15 2898
Product name :
Ferritin 1-2 (plant)
Immunogen :
Purified ferritin from dried peas, Pisum sativum L. After extraction from pea flour, the ferritin was further purified by gel filtration chromatography to >95% purity as estimated from a Coomassie-stained gel.Antibody is most likely to bind to all ferritin isoforms from pea however it has not been confirmed as yet.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS10 674 Anti- Ferritin (plant), rabbit antibody (made to synthetic peptide), rabbit antibodiesAS06 126 Anti-Fer1 ferritin 1 (pre-apoferritin, algae), rabbit antibodiesPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 5000-10 000 (WB)
Expected apparent MW :
23 kDa in legumes, 24 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Brassica oleracea, Hordeum vulgare, Medicago truncatula, Pisum sativum, Spinacia oleracea
Predicted reactivity :
Species of your interest not listed? Contact us
Additional information :
Note, the calculated molecular weight of pea ferritin is 28 kDa. Removal of the N-terminal targeting sequence upon protein import into plastids results in a protein with an apparent mol weight of ~23 kDa.This antibody is also recognizing horse ferritin (above 100 ng in Western blot).
Selected references :
Jiang et al. (2022) Reactive effects of pre-sowing magnetic field exposure on morphological characteristics and antioxidant ability of Brassica juncea in phytoextraction. Chemosphere. 2022 Sep;303(Pt 1):135046. doi: 10.1016/j.chemosphere.2022.135046. Epub 2022 May 23. PMID: 35618056.Bastow et al. (2018). Vacuolar Iron Stores Gated by NRAMP3 and NRAMP4 Are the Primary Source of Iron in Germinating Seeds. Plant Physiol. 2018 Jul;177(3):1267-1276. doi: 10.1104/pp.18.00478.
Background :
Ferritin forms a 24-subunit cage for storage of mineral iron. In plants, ferritin is predominantly localized in the plastids, and its expression is upregulated in response to iron or oxidative stress.
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
Research area :
Arabidopsis thaliana
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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