Lhcb2-P LHCII type II chlorophyll a/b-binding protein, phosphorylated

product summary

company name :

Agrisera

product type :

antibody

product name :

Lhcb2-P LHCII type II chlorophyll a/b-binding protein, phosphorylated

catalog :

AS13 2705

quantity :

25 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; fig 1	Longoni P, Douchi D, Cariti F, Fucile G, Goldschmidt Clermont M. Phosphorylation of the Light-Harvesting Complex II Isoform Lhcb2 Is Central to State Transitions. Plant Physiol. 2015;169:2874-83 pubmed publisher

product information

Product number :

AS13 2705

Product name :

Lhcb2-P LHCII type II chlorophyll a/b-binding protein, phosphorylated

Immunogen :

KLH-conjugated synthetic peptide: RRT*VKSTPQS, where T* indicates phospho-Thr

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

25 µg

Tested applications :

Western blot (WB)

Related products :

AS01 003 anti-Lhcb2 LHCII type II chlorophyll a/b-binding protein, rabbit antibodiesAS13 2704 anti-Lhcb1-P LHCII type I chlorophyll a/b-binding protein, phopshorylated, rabbit antibodiesAll anti-LHC antibodiesAntibodies to other proteins involved in photosynthesisPlant protein extraction buffer Secondary antibodies

Recommended dilutions :

1 : 10 000 (WB)

Expected apparent MW :

25 25 kDa for Arabidopsis thaliana

Confirmed reactivity :

Arabidopsis thaliana, Echinochloa crus-galli, Asterochloris erici (lichen photobiont), Zea mays

Predicted reactivity :

Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Solanum lycopersicum, Spinacia oleracea, Physcomitrella patens

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Selected references :

Gasulla et al. (2018). Chlororespiration induces non-photochemical quenching of chlorophyll fluorescence during darkness in lichen chlorobionts. Physiol Plant. 2018 Jun 27. doi: 10.1111/ppl.12792. Rantala and Tikkanen et al. (2018). Phosphorylationâ€induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2. Rantala et al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732. Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218. Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21.Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.

Pubmed ID's :

29952012, 28980426, 25896488, 23888908

Background :

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.

Reconstitution :

For reconstitution add 25 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

more info or order :

Agrisera product webpage