antibody

Lhcb1-P LHCII type I chlorophyll a/b-binding protein, phopshorylated

AS13 2704

25 µg

polyclonal

rabbit

nonconjugated

Lhcb1-P LHCII type I chlorophyll a/b-binding protein, phopshorylated

KLH-conjugated synthetic peptide RKT*VAKPKGP, where T* indicates phospho-Thr

Rabbit

Polyclonal

Affinity purified serum in PBS, pH 7.4

Lyophilized

25 µg

Western blot (WB)

AS01 004 Lhcb1 LHCII type I chlorophyll a/b-binding protein , rabbit antibodies AS13 2705 Lhcb2 LHCII type II chlorophyll a/b-binding protein, phosphorylated, rabbit antibodiesAll anti-LHC antibodiesAntibodies to other proteins involved in photosynthesisPlant and algal protein extraction buffer Secondary antibodies

1 : 10 000 (WB)

25 25 kDa for Arabidopsis thaliana

Arabidopsis thaliana

Arachis hypogaea, Colobanthus quitensis Kunt Bartl, Hordeum vulgare, Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Silene vulgaris, Solanum lycopersicum, Spinacia oleracea, Zea mays

No confirmed exceptions from predicted reactivity are currently known.

Rantala and Tikkanen et al. (2018). Phosphorylation‐induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2. Rantala et al. (2017). Proteomic characterization of hierarchical megacomplex formation in Arabidopsis thylakoid membrane. Plant J. 2017 Dec;92(5):951-962. doi: 10.1111/tpj.13732. Fristedt et al. (2017). PSB33 sustains photosystem II D1 protein under fluctuating light conditions. Journal of Experimental Botany doi:10.1093/jxb/erx218. Schönberg et al. (2017). Identification of STN7/STN8 kinase targets reveals connections between electron transport, metabolism and gene expression. Plant J. 2017 Jun;90(6):1176-1186. doi: 10.1111/tpj.13536. Longoni et al. (2015). Phosphorylation of the Lhcb2 isoform of Light Harvesting Complex II is central to state transitions. Plant Physiol. 2015 Oct 5. pii: pp.01498.2015. Sato et al. (2015). Chlorophyll b degradation by chlorophyll b reductase under high-light conditions. Photosynth Res. 2015 Apr 21. Jia et al. (2014). Accumulation of NON-YELLOW COLORING 1 protein of the chlorophyll cycle requires chlorophyll b in Arabidopsis thaliana. Plant J. 2014 Dec 30. doi: 10.1111/tpj.12753.Leoni et al. (2013). Very rapid phosphorylation kinetics suggest a unique role for Lhcb2 during state transitions in Arabidopsis. Plant J. Oct;76(2):236-46. doi: 10.1111/tpj.12297. Epub 2013 Aug 26.

28980426, 28295753, 26438789, 25896488, 25557327, 23888908

The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes.

For reconstitution add 25 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.