product summary
Loading...
company name :
Agrisera
product type :
antibody
product name :
AGO2 Argonaute 2
catalog :
AS13 2682
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS13 2682
Product name :
AGO2 Argonaute 2
Immunogen :
KLH-conjugated synthetic peptide, derived from Arabidopsis thaliana AGO2 protein, UniProt: Q9SHF3, TAIR: AT1G31280
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS09 527 Anti-AGO1 argonaute 1, rabbit antibodyAS09 527-ALP Anti-AGO1 argonaute 1 (40 µg, ALP-conjugated) AS09 527B Anti-AGO1 argonaute 1 (40 µg, Biotin conjugated) AS09 527-HRP Anti-AGO1 argonaute 1 (40 µg, HRP-conjugated) AS14 2776 Anti-AGO1 argonaute 1 (Chlamydomonas), rabbit antibodyAS09 527P AGO1 argonaute 1 Blocking peptideAS09 617 Anti-AGO4 argonaute 4, rabbit antibodyAS10 671 Anti-AGO5 argonaute 5, rabbit antibodyAS10 672 Anti-AGO6 argonaute 6, rabbit antibodyAS10 673 Anti-AGO9 argonaute 9, rabbit antibodyAS15 3071 Anti-AGO10 argonaute 10, rabbit antibodycollection of antibodies to micro RNAPlant protein extraction buffer
Recommended dilutions :
1 : 250-1 : 500 (WB)
Expected apparent MW :
113 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Capsella rubella, Solanum tuberosum
Not reactive in :
Chlamydomonas reinhardtii, Solanum lycopersicum, Zea mays
Additional information :
AGO2 protein is strongly induced by stress. AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure. Antibody incubation should be done over night in 4°C. Use of material with enriched AGO2 levels is recommened.
Background :
AGO2 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC). This protein complex is responsible for the gene silencing (RNAi). AGO2 is probably involved in antiviral RNA silencing.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q9SHF3
TAIR number :
AT1G31280
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
browse more products
questions and comments