product summary
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company name :
Agrisera
product type :
antibody
product name :
MKKK18 Mitogen-activated protein kinase 18
catalog :
AS13 2673
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress, Escherichia coli
application :
western blot, immunocytochemistry
more info or order :
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; thale cress; 1:5000; fig 6
  • western blot; Escherichia coli; 1:5000; fig 6
Mitula F, Tajdel M, Cieśla A, Kasprowicz Maluśki A, Kulik A, Babula Skowrońska D, et al. Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015;56:2351-67 pubmed publisher
product information
Product number :
AS13 2673
Product name :
MKKK18 Mitogen-activated protein kinase 18
Immunogen :
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana kinase 18 sequence UniProt: Q9ZVP5, TAIR: AT1G05100
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Western blot (WB)
Related products :
Plant and algal protein extraction buffer
Recommended dilutions :
3 µl (IP), 1 : 1000 (WB)
Expected apparent MW :
37.7 38 kDa
Confirmed reactivity :
Arabidopsis thaliana
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
MKKK18 is not stable in endogenous extracts and to allow succesfull detection use transgenic plants or transient expression in protoplasts.
Selected references :
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Pubmed ID's :
26443375
Background :
Mitogen-activated protein kinase 18 has ATP binding and serine/threonine kinase activity. Alternative names: T7A14.2 protein, Putative NPK1-related MAP kinase.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
TAIR number :
AT1G05100
more info or order :
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.