product summary
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company name :
Agrisera
product type :
antibody
product name :
H+ATPase Plasma membrane H+ATPase (chicken antibody)
catalog :
AS13 2671
quantity :
50 µg
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS13 2671
Product name :
H+ATPase Plasma membrane H+ATPase (chicken antibody)
Immunogen :
KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)
Host :
Chicken
Clonality :
Polyclonal
Purity :
Affinity purified IgY
Format :
Lyophilized in PBS pH 7.4
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
AS07 260 H+ATPase plasma membrane H+ATPase, rabbit antibodyantibodies to membrane transport systemrecommended secondary antibody
Recommended dilutions :
1 : 1000-1 : 5000 (WB)
Expected apparent MW :
95 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Spinacia oleracea, Zea mays
Predicted reactivity :
Angomonas deanei, Avena sativa, Brassica napus, Citrus limon, Coffea canephora, Cucumis sativus, Cucurbita moschata, Dunaliella spp, Eichhornia crassipes, Emiliana huxleyi, Glycine max (weak), Hordeum vulgare, Lactobacillus johnsonii, Laishamania braziliensis, Nicotiana tabacum, Oryza sativa, Solanum lycopersicon, Solanum tuberosum, Medicago truncatula, Mesembruanthemum crystallinum), Nepenthes alata, Nicotiana tabaccum, Nitrospira bacterium, Oryza sativa, Ostreococcus spp., Phaseolus acutifolius, Physocomitrella patens, Picea abies, Pinus thunbergii, Populus tremula, Pteris vittata, Ricinus communis, Saccharomyces cerevisiae, Solanum lycopersicum, Strigomonas culicis, Toxoplasma gondii, Triticum urartu, Trypanosoma cruzi, Zosteria marina, Vicia faba, Vigna angularis
Not reactive in :
Chlamydomonas reinhardtii
Additional information :
VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.
Selected references :
to be added when available, antibody released in November 2014
Background :
The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Cellular [compartment marker] for plasma membranetissue specific immunolocalization was done on paraffin emdedded samples as described here.
UniProt number :
Q9FNS3
TAIR number :
At2g18960
more info or order :
company information

Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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