antibody

H+ATPase Plasma membrane H+ATPase (chicken antibody)

AS13 2671

50 µg

polyclonal

chicken

nonconjugated

H+ATPase Plasma membrane H+ATPase (chicken antibody)

KLH-conjugated synthetic peptide derived from available di and monocot, fern, mosses and algal plasma membrane ATPase sequences including Arabidopsis thaliana ATPase 1 (At2g18960) and ATPase 2,3,4,6,7,8,9 of Arabidopsis thaliana and hydrogen ATPase of Chlamydomonas reinhardtii (Q9FNS3)

Chicken

Polyclonal

Affinity purified IgY

Lyophilized in PBS pH 7.4

50 µg

Western blot (WB)

AS07 260 H+ATPase plasma membrane H+ATPase, rabbit antibodyantibodies to membrane transport systemrecommended secondary antibody

1 : 1000-1 : 5000 (WB)

95 kDa (Arabidopsis thaliana)

Arabidopsis thaliana, Spinacia oleracea, Zea mays

Angomonas deanei, Avena sativa, Brassica napus, Citrus limon, Coffea canephora, Cucumis sativus, Cucurbita moschata, Dunaliella spp, Eichhornia crassipes, Emiliana huxleyi, Glycine max (weak), Hordeum vulgare, Lactobacillus johnsonii, Laishamania braziliensis, Nicotiana tabacum, Oryza sativa, Solanum lycopersicon, Solanum tuberosum, Medicago truncatula, Mesembruanthemum crystallinum), Nepenthes alata, Nicotiana tabaccum, Nitrospira bacterium, Oryza sativa, Ostreococcus spp., Phaseolus acutifolius, Physocomitrella patens, Picea abies, Pinus thunbergii, Populus tremula, Pteris vittata, Ricinus communis, Saccharomyces cerevisiae, Solanum lycopersicum, Strigomonas culicis, Toxoplasma gondii, Triticum urartu, Trypanosoma cruzi, Zosteria marina, Vicia faba, Vigna angularis

Chlamydomonas reinhardtii

VERY IMPORTANT: please, do not heat up your samples over 70°C as this might cause H+ATPase to precipitate and there will be no signal on your western blot.

to be added when available, antibody released in November 2014

The Plasma Membrane H+ATPase is a family of proteins of ca. 100 kDa that are believed to be exclusive to the plasma membranes of plants and fungi. The protein is anchored within biological membrane which creates an electrochemical gradient used as an energy source and is essential for uptake of most metabolites and plant responses to environment, for example movement of leaves.

For reconstitution add 50 µl of sterile water.

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Cellular [compartment marker] for plasma membranetissue specific immunolocalization was done on paraffin emdedded samples as described here.

Q9FNS3

At2g18960