PsbA D1 protein of PSII, phosphorylated | Agrisera AS13 2669 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PsbA D1 protein of PSII, phosphorylated

catalog :

AS13 2669

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

product information

Product number :

AS13 2669

Product name :

PsbA D1 protein of PSII, phosphorylated

Immunogen :

KLH-conjugated synthetic peptide derived from available plant PsbA sequences with phosphorylated (T), including Arabidopsis thaliana UniProt: P83755, TAIR:AtCg00020, Oryza sativa P0C434 and other higher plant PsbA sequences

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

AS05 084 PsbA D1 protein of PSII, C-terminal, rabbit antibodyAS05 084PRE PsbA D1 protein of PSII, C-terminal, pre-immune serumAS01 016 PsbA C-terminal, chicken antibodyAS11 1786 PsbA N-terminal, rabbit antibodyAS01 016S PsbA protein standard for quantitation and positive controlAS10 704 PsbA D1 protein of PSII, DE-loop, rabbit antibodyPlant and algal protein extraction buffer

Recommended dilutions :

1 : 10 000 (WB)

Expected apparent MW :

38 28-30 kDa

Confirmed reactivity :

Arabidopsis thaliana, Hordeum vulgare, Zea mays

Predicted reactivity :

Dictos, Conifers, Chlamydomonas reinhardtii, Monocts

Not reactive in :

Cyanobacteria

Additional information :

This antibody is detecting phosphorylated PsbA protein.Antibodies are purified on a non-phosphorylated peptide.

Selected references :

Xing et al. (2017). Deletion of CGLD1 Impairs PSII and Increases Singlet Oxygen Tolerance of Green Alga Chlamydomonas reinhardtii. Front. Plant Sci., 15 December 2017.Li et al. (2015). Effect of hydrogen sulfide on D1 protein in wheat under drought stress. Acta Physiologiae Plantarum November 2015, 37:225.

Background :

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

P83755

TAIR number :

AtCg00020

more info or order :

Agrisera product webpage