product summary
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company name :
Agrisera
product type :
antibody
product name :
ACT Actin
catalog :
AS13 2640
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunocytochemistry
more info or order :
Agrisera product webpage
citations: 5
Reference
Chen C, Shu J, Li C, Thapa R, Nguyen V, Yu K, et al. RNA polymerase II-independent recruitment of SPT6L at transcription start sites in Arabidopsis. Nucleic Acids Res. 2019;47:6714-6725 pubmed publisher
Brandt S, Fachinger S, Tohge T, Fernie A, Braun H, Hildebrandt T. Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLoS ONE. 2018;13:e0202153 pubmed publisher
Minina E, Moschou P, Vetukuri R, Sanchez Vera V, Cardoso C, Liu Q, et al. Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness. J Exp Bot. 2018;69:1415-1432 pubmed publisher
Wei Y, Caceres Moreno C, Jimenez Gongora T, Wang K, Sang Y, Lozano Durán R, et al. The Ralstonia solanacearum csp22 peptide, but not flagellin-derived peptides, is perceived by plants from the Solanaceae family. Plant Biotechnol J. 2018;16:1349-1362 pubmed publisher
Buxa S, Degola F, Polizzotto R, De Marco F, Loschi A, Kogel K, et al. Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. Front Plant Sci. 2015;6:650 pubmed publisher
product information
Product number :
AS13 2640
Product name :
ACT Actin
Immunogen :
ca. 100 amino acids of recombinant actin conserved more than 80 % in Arabidopsis thaliana: actin-1 P0CJ46 AT2G37620, actin-2 Q96292 AT3G18780, actin-3 P0CJ47 AT3G53750, actin-4 P53494 AT5G59370, actin-5 Q8RYC2 At2g42100, actin-7 P53492 At5g09810, actin-8 Q96293 AT1G49240 , actin-11 P53496 , AT3G12110 ,actin-12 P53497 AT3G46520
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
immunofluorescence (IF), Western blot (WB)
Related products :
AS10 702 Anti-Actin-11, monclonal mouse antibody AS10 681 Anti-tubulin beta chain, rabbit antibodyAS10 680 Anti-tubulin alpha chain, rabbit antibodyAS16 3140 Actin-2, mouse monoclonal antibodyAS16 3141 Actin-8, mouse monoclonal antibodyAS16 3139 Actin-1, monoclonal antibodyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 250 (IF), 1 : 3000-1 : 5000 (WB)
Expected apparent MW :
41.6 45 kDa
Confirmed reactivity :
Agostis stolonifera cv. ‘Penncross’, Arabidopsis thaliana, Cucumis sativus, Cynara cardunculus, Glycine max, Hordeum vulgare, Nicotiana tabacum, Solanum tuberosum, Zea mays
Predicted reactivity :
Agropyron cristatum, Beta vulgaris, Betula luminifera, Brassica napus, Brassica rapa subsp. pekinensis, Capsella rubella, Castanea sativa, Chorispora bungeana, Cyanidioschyzon merolae strain 10D, Glycine max, Glycine soja, Halogeton glomeratus, Medicago truncatula, Malus domestica, Oryza sativa, Pisum sativun, Solanum lysopersicum, Solanum tuberosum, Phaseolus vulgaris, Picea abies, Picea sitchensis, Prunus avium, Ricinus communis, Rubus plicatus, Theobroma cacao, Triticum aestivum, Vicia faba
Not reactive in :
Chlamydomonas reinhardtii (too high background for this species)
Additional information :
This product can be sold containing ProClin if requested
Selected references :
López‐Calcagno et al. (2018). Overexpressing the H‐protein of the glycine cleavage system increases biomass yield in glasshouse and field‐grown transgenic tobacco plants. Plant Biotechnology Journal, May 2018. Li et al. (2018). Comparative proteomic analysis of key proteins during abscisic acid-hydrogen peroxide-induced adventitious rooting in cucumber (Cucumis sativus L.) under drought stress. Journal of Plant Physiology Volume 229, October 2018, Pages 185-194. Adhikari et al. (2018). Sulfate improves cadmium tolerance by limiting cadmium accumulation, modulation of sulfur metabolism and antioxidant defense system in maize. Environmental and Experimental Botany Volume 153, September 2018, Pages 143-162. Brandt et al. (2018). Extended darkness induces internal turnover of glucosinolates in Arabidopsis thaliana leaves. PLoS One. 2018 Aug 9;13(8):e0202153. doi: 10.1371/journal.pone.0202153. Jespersen et al. (2017). Metabolic Effects of Acibenzolar-S-Methyl for Improving Heat or Drought Stress in Creeping Bentgrass. Front Plant Sci. 2017 Jul 11;8:1224. doi: 10.3389/fpls.2017.01224. eCollection 2017. (western blot, Agostis stolonifera cv. ‘Penncross’) Qiu et al. (2015). Soy 14-3-3 protein SGF14c, a new regulator of tolerance to salt–alkali stress. Plant Biotechnology Reports pp 1-9.Shaw et al. (2015). beta-aminobutyric acid mediated drought stress alleviation in maize (Zea mays L.). Environ Sci Pollut Res Int. 2015 Sep 29. Buxa et al. (2015). Phytoplasma infection in tomato is associated with re-organization of plasma membrane, ER stacks, and actin filaments in sieve elements. ront Plant Sci. 2015; 6: 650. Published online 2015 Aug 19. Zheng et al. (2014). iTRAQ-based quantitative proteomics analysis revealed alterations of carbohydrate metabolism pathways and mitochondrial proteins in a male sterile cybrid pummelo. J Proteome Res. 2014 May 13.
Pubmed ID's :
30092103, 28744300, 26416125, PMC4541602, 24824475
Background :
Actin is a highly conserved protein and an essential component of cell cytoskeleton and plays an important role in cytoplasmic streaming, cell shape determination, cell division, organelle movement and extension growth. Preferentially expressed in young and expanding tissues, floral organ primordia, developing seeds and emerging inflorescence.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody available in 3 various pack sizes: 50, 100 and 150 µl - please, inquire. This product can be sold containing PrClin if requested.
UniProt number :
P0CJ46 , Q96292 , P0CJ47 , P53494 , Q8RYC2 , P53492 , Q96293 , P53496 , P53497
TAIR number :
AT2G37620, AT3G18780, AT3G53750, AT5G59370, At2g42100, At5g09810, AT1G49240, AT3G12110, AT3G46520
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
https://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.