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company name :
Agrisera
product type :
antibody
product name :
NRT2,1 Nitrate transporter 2,1
catalog :
AS12 2612
quantity :
50 µg
price :
302 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS12 2612
Product name :
NRT2,1 Nitrate transporter 2,1
Quantity :
50 µg
List Price :
302 USD
Special price 2+ units (List Price) :
226
Special price 3+ units (List Price) :
205
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NRT2.1 C-terminal sequence, UniProt: O82811, TAIR:AT1G08090
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Immunogen affinity purified serum in PBS pH 7.4.
Format :
Lyophilized
Tested applications :
Western blot (WB)
Recommended dilutions :
1 : 5000 (WB)
Expected apparent MW :
57,7 45 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Brassica juncea, Brassica napus, Brassuca rapa, Ricinus communis
Not reactive in :
Alexandrium pacificum, Solanum tuberosum
Additional information :
Microsomal fraction is recommended to be used as it will have higher amount of NRT2,1 protein, If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail
Related products :
Plant protein extraction buffer Secondary antibodies
Selected references :
Zou et al. (2019). Phosphorylation at Ser28 stabilizes the Arabidopsis nitrate transporter NRT2.1 in response to nitrate limitation. J Integr Plant Biol. 2019 Jul 24. doi: 10.1111/jipb.12858.
Background :
NRT2.1 is a high affinity nitrate transporter which is up-regulated by nitrate. Functions as a repressor of lateral root initiation independently of nitrate uptake. Synonymes: Protein ACH1, Protein LATERAL ROOT INITIATION 1, ACH1, ATNRT2.1, ATNRT2:1,, LIN1, NITRATE TRANSPORTER 2, NITRATE TRANSPORTER 2.1, NITRATE TRANSPORTER 2:1, NRT2, NRT2.1, NRT2:1, NRT2;1AT
Reconstitution :
For reconstitution add 50 µl of sterile water
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
UniProt number :
O82811
TAIR number :
AT1G08090
Commodity Code :
38220000
Our Category :
Plant Signal Transduction
Research area :
Cell Signaling
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.