product summary
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company name :
Agrisera
product type :
antibody
product name :
DCL3 Dicer-like protein 3
catalog :
AS12 2103
quantity :
200 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS12 2103
Product name :
DCL3 Dicer-like protein 3
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana DCL3 sequence, Q9LXW7 , At3g43920
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
200 µg
Tested applications :
Western blot (WB)
Related products :
AS12 2102 anti-DCL1 Dicer-like protein 1, rabbit antibodiesAS15 3100 anti-DCL2 Endoribonuclease Dicer homolog 2, rabbit antibodiesAS15 3105 anti-DCL4 Dicer-like protein 4, rabbit antibodiescollection of antibodies to proteins involved in microRNA metabolismPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
117 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
Zea mays
Additional information :
Recommend protein load is from 40-50 µg/well (using 1.5 mm spacers helps to obtain wider wells)
Selected references :
to be added when available, antibody released in August 2012.
Background :
DCL3 (EC=3.1.26) is a ribonuclease (RNase) III involved in RNA-mediated post-transcriptional gene silencing (PTGS). Functions in the microRNAs (miRNAs) biogenesis pathway by cleaving primary miRNAs (pri-miRNAs) and precursor miRNAs (pre-miRNAs). Synonymes: Endoribonuclease Dicer homolog 3.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q9LXW7
TAIR number :
AT3G43920
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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