ABI2 Abscisic acid insensitive 2 | Agrisera AS12 1871 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

ABI2 Abscisic acid insensitive 2

catalog :

AS12 1871

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; fig 7	Mitula F, Tajdel M, CieÅ›la A, Kasprowicz MaluÅ›ki A, Kulik A, Babula SkowroÅ„ska D, et al. Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015;56:2351-67 pubmed publisher

product information

Product number :

AS12 1871

Product name :

ABI2 Abscisic acid insensitive 2

Immunogen :

KLH-conjugated synthetic peptide derived from N-terminus of Arabidopsis thaliana ABI2 sequence, UniProt: O04719, ,TAIR: AT5G57050 chosen peptide is not conserved in ABI1

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum

Format :

Lyophilized in PBS pH 7.4

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

AS12 1861 ABI1 abscisic acid insensitive 1, rabbit antibody AS12 1862 ABI3 abscisic acid insensitive 3, rabbit antibodyAS12 1863 ABI5 abscisic acid insensitive 5, rabbit antibodyAS13 2634 PYR1 Abscisic acid receptor RCAR11, rabbit antibodyPlant protein extraction buffer Secondary antibodies

Expected apparent MW :

46 kDa

Confirmed reactivity :

Arabidopsis thaliana

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

ABI2 antibodies recognize recombiant StrepTag-ABI2, ABI2-GST, His-ABI2. ABI2 protein is easily degraded therefore extraction buffer needs to contain protease inhibotors, example of such inhibitor coctail can be found here.

Selected references :

Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.

Pubmed ID's :

26443375

Background :

Protein Abscisic acid-insensitive 2 (ABI2) is a represor of the ABA signaling pathway, regulating various ABA responses like: stomatal closure, osmotic water permability of the plasma membrane, high light stress, response to glucose, seed germination and inhibition of vegetative growth. Involved in acquired thermotolerance. Alternative names: ABA INSENSITIVE 2, ABI2, ATABI2, Protein phosphatase 2C 77, AtPP2C77, At5g57050, MHM17_19, MHM17.19, PP2C ABI2.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

To detect endogenous ABI2 plant material needs to be subjected to stress before harvesting.

UniProt number :

O04719

TAIR number :

AT5G57050

more info or order :

Agrisera product webpage