product summary
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company name :
Agrisera
product type :
antibody
product name :
HY5 Protein long hypocotyl 5
catalog :
AS12 1867
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS12 1867
Product name :
HY5 Protein long hypocotyl 5
Immunogen :
KLH-conjugated peptide, derived from Arabidopsis thaliana HY5 protein sequence, UniProt:O24646 , TAIR: AT5G11260. Chosen peptide is not conserved in HYH protein sequence.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Western blot (WB)
Related products :
Collection of antibodies to proteins involved in photomorphogenesisPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1: 500 to 1 : 1000 (WB)
Expected apparent MW :
18.5 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Brassica pekinensis
Not reactive in :
Hordeum vulgare, Oryza sativa, Pisum sativum, Populus sp. , Solanum lycopersicum, Triticum aestivum
Additional information :
This antibody detects well recombinant HY5 in Nicotiana benthamiana (image below). Urea buffer needs to be used when working with endogenous extract to allow detection with this antibody.
Selected references :
Lee et al. (2017). The F-box protein FKF1 inhibits dimerization of COP1 in the control of photoperiodic flowering. Nat Commun. 2017 Dec 22;8(1):2259. doi: 10.1038/s41467-017-02476-2.
Pubmed ID's :
29273730
Background :
HY5 (Protein long hypocotyl 5) is a light-regulated transcription factor that promotes photomorphogenesis in light. Involved in the blue light specific pathway. Heterodimer. Expressed in root, hypocotyl, cotyledon, leaf, stem and floral organs. Localized in nucleus. Alternative names: Protein LONG HYPOCOTYL 5, bZIP transcription factor 56, AtbZIP56.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
O24646
TAIR number :
AT5G11260
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.