product summary
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company name :
Agrisera
product type :
antibody
product name :
ABI1 Abscisic acid insensitive 1
catalog :
AS12 1861
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunoprecipitation
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
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product information
Product number :
AS12 1861
Product name :
ABI1 Abscisic acid insensitive 1
Immunogen :
KLH-conjugated peptide, derived from Arabidopsis thaliana ABI1 sequence UniProt: P49597, TAIR: AT4G26080. Chosen peptide is not present in AtABI2.
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Related products :
AS12 1871 ABI2 abscisic acid insensitive 2, rabbit antibodyAS12 1862 ABI3 abscisic acid insensitive 3, rabbit antibodyAS12 1863 ABI5 abscisic acid insensitive 5, rabbit antibodyAS13 2634 PYR1 Abscisic acid receptor RCAR11, rabbit antibodyAS13 2635 SRK2E Ser/Thr-protein kinase SnRK2.6, rabbit antibodyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
5 µg (IP for a 200 ul of a cell extract), 3 µg (WB)
Expected apparent MW :
47.5 kDa
Confirmed reactivity :
Arabidopsis thaliana
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Important note: blocking with more than 3 % skimmed milk will result in lack of signal for this antibody.
Selected references :
Mitula et al. (2015). Arabidopsis ABA-Activated Kinase MAPKKK18 is Regulated by Protein Phosphatase 2C ABI1 and the Ubiquitin-Proteasome Pathway. Plant Cell Physiol. 2015 Dec;56(12):2351-67. doi: 10.1093/pcp/pcv146. Epub 2015 Oct 6.
Pubmed ID's :
26443375
Background :
ABI1 is a key component and repressor of the abscisic acid (ABA) signaling pathway. It regulates numerous ABA responses, such as stomatal closure, osmotic water permeability of the plasma membrane, drought-induced resistance and rhizogenesis, response to glucose, high light stress, seed germination and inhibition of vegetative growth. Expressed in seeds and seedlings. Confined to lateral root caps and columella cells in roots. Induced by low temperature, drought, high salt, ABA and ethylene. Activates/represses SnRK2.6/SRK2E/OST1 in response to ABA-dependent stimuli. Alternative names: ABI1, ABA INSENSITIVE 1, AtABI1, Protein phosphatase 2C 56, AtPP2C56, PP2C56.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
It is of crucial importance to chose a material in which ABI1 protein is highly expressed like seeds or senescent leaf. This protein could not be detected using this antibody in plants grown under optimal conditions. The antibody detects both, recombinant and endogenous ABI1 protein.
UniProt number :
P49597
TAIR number :
AT4G26080
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.