product summary
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company name :
Agrisera
product type :
antibody
product name :
BRI1 Brassinosteroid insensitive 1
catalog :
AS12 1859
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunoprecipitation
more info or order :
product information
Product number :
AS12 1859
Product name :
BRI1 Brassinosteroid insensitive 1
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized in PBS pH 7.4.
Quantity :
50 µg
Tested applications :
Immunoprecipitation (IP), Western blot (WB)
Related products :
AS12 1858 anti-BAK1 Brassinosteroid insensitive 1-associated receptor kinase 1, rabbit antibodiesAS16 3203 Anti-BIN2 brassinosteroid insensitive 2 antibodyAS16 3204 Anti-SOBIR1 supressor of BIR1 antibodyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 5000 (WB)
Expected apparent MW :
above 130 kDa (due to N-glycosylation)
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Brassica rapa
Not reactive in :
Hordeum vulgare, Oryza sativa
Additional information :
Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 µl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.Protein extraction has to be done efficiently as this step is crucial, recommended material to buffer ratio: 15 µl/µg or less.
Selected references :
to be added when available, antibody released in July 2014.
Background :
BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
O22476
TAIR number :
AT4G39400
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.