BAK1 Brassinosteroid insensitive 1-associated receptor kinase 1

product summary

company name :

Agrisera

product type :

antibody

product name :

BAK1 Brassinosteroid insensitive 1-associated receptor kinase 1

catalog :

AS12 1858

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

Arabidopsis, thale cress

application :

western blot, immunoprecipitation

more info or order :

Agrisera product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; Arabidopsis; 1:3000; fig 2d	Cecchini N, Jung H, Engle N, Tschaplinski T, Greenberg J. ALD1 Regulates Basal Immune Components and Early Inducible Defense Responses in Arabidopsis. Mol Plant Microbe Interact. 2015;28:455-66 pubmed publisher

product information

Product number :

AS12 1858

Product name :

BAK1 Brassinosteroid insensitive 1-associated receptor kinase 1

Immunogen :

KLH-conjugated peptide, chosen from Arabidopsis thaliana Bak1 sequence, TAIR: AT4G33430 UniProt: Q94F62

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized in PBS pH 7.4.

Quantity :

50 µg

Tested applications :

Immunoprecipitation (IP), Western blot (WB)

Related products :

AS12 1859 anti-BRI1 BRASSINOSTEROID INSENSITIVE 1AS12 1857 anti-FLS2 FLAGELLIN-SENSITIVE 2AS16 3203 Anti-BIN2 brassinosteroid insensitive 2 antibodyAS16 3204 Anti-SOBIR1 supressor of BIR1 antibodyPlant protein extraction buffer Secondary antibodies

Recommended dilutions :

2 µl/50 µl of Protein G agarose, 1 : 5000 (WB)

Expected apparent MW :

68 70 kDa

Confirmed reactivity :

Arabidopsis thaliana, Solanum lycopersicum

Predicted reactivity :

Thelungiella halophila

Not reactive in :

Hordeum vulgare, Nicotiana benthamiana, Oryza sativa

Additional information :

Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.

Selected references :

Hu et al. (2018). A group of receptor kinases are essential for CLAVATA signalling to maintain stem cell homeostasis. Nat Plants. 2018 Apr;4(4):205-211. doi: 10.1038/s41477-018-0123-z. (CoIP)Bundy et al. (2016). A mutation in the catalytic subunit of the glycosylphosphatidyl inositol transamidase disrupts growth, fertility and stomata forma formation in Arabidopsis. Plant Physiology. DOI:10.1104/pp.16.00339.Tateda et al. (2014). Salicylic Acid Regulates Arabidopsis Microbial Pattern Receptor Kinase Levels and Signaling.Plant Cell. 2014 Oct 14. pii: tpc.114.131938.

Pubmed ID's :

29581511, 25315322

Background :

BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD). Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

Q94F62

TAIR number :

AT4G33430

more info or order :

Agrisera product webpage