S6K1/2 Ribosomal S6 kinase 1/2 | Agrisera AS12 1855 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

S6K1/2 Ribosomal S6 kinase 1/2

catalog :

AS12 1855

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; fig s1	Pfeiffer A, Janocha D, Dong Y, Medzihradszky A, Schöne S, Daum G, et al. Integration of light and metabolic signals for stem cell activation at the shoot apical meristem. elife. 2016;5: pubmed publisher

product information

Product number :

AS12 1855

Product name :

S6K1/2 Ribosomal S6 kinase 1/2

Immunogen :

KLH-conjugated peptide, derived from Arabidopsis thaliana S6K1: UniProt: P42818, TAIR: AT3G08730 and S6K2: UniProt: Q39030, TAIR: AT3G08720. Due to high amino acid homology, chosen peptide is conserved in both proteins: S6K1 and S6K2.

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4 and 0.02% sodium azide.

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

AS12 2608 TOR Target of rapamycin, rabbit antibodyAS13 2664 S6K1-2 Ribosomal-protein S6 kinase homolog 1,2 - phosphorylated, rabbit antibodyPlant protein extraction buffer Secondary antibodies

Recommended dilutions :

1 : 750-1 : 1000 (WB)

Expected apparent MW :

52.6 kDa (S6K1) and 53 kDa (S6K2)

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Thelungiella halophila

Not reactive in :

Setaria viridis

Additional information :

Keeping the samples at 4°C all times is of crucial importance. Laucs buffer was used in example below since this is the one which was used routinely by this test laboratory for the work with kinases. Some unspecific bands can be seen, depending upon western blot protocol which is used. Therefore please consider to use a negative control together with your samples.

Selected references :

Inaba and Nagy (2018). Tombusvirus RNA replication depends on the TOR pathway in yeast and plants. Virology. 2018 Jun;519:207-222. doi: 10.1016/j.virol.2018.04.010. Pfeiffer et al. (2016). Integration of light and metabolic signals for stem cell activation at the shoot apical meristem. Elife. 2016 Jul 11;5. pii: e17023. doi: 10.7554/eLife.17023.

Pubmed ID's :

29734044, 27400267

Background :

Ribosomal S6 kinase 1/2 (S6K1/2) involved in TOR signaling pathway, in osmotic stress response. Activated by PDK1 and repressed during osmotic stress. Expressed in all tissues, especially during high metabolic activity in growing buds, root tips, leaf margins and germinating seeds. Alternative names: AtPK1/AtPK6 S6K1), AtPK2/AtPK19 (S6K2).

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

So far in applied conditions and extracts endogenous S6K1/2 protein is detectable as a very weak band.

UniProt number :

P42818 , Q39030

TAIR number :

AT3G08730, AT3G08720

more info or order :

Agrisera product webpage