product summary
Loading...
company name :
Agrisera
product type :
antibody
product name :
NpHR Halorhodopsin
catalog :
AS12 1851
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Natronomonas pharaonis DSM 2160, mouse
application :
western blot, immunocytochemistry
more info or order :
Agrisera product webpage
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; mouse; 1:200; fig 2
Alfonsa H, Merricks E, Codadu N, Cunningham M, Deisseroth K, Racca C, et al. The contribution of raised intraneuronal chloride to epileptic network activity. J Neurosci. 2015;35:7715-26 pubmed publisher
product information
Product number :
AS12 1851
Product name :
NpHR Halorhodopsin
Immunogen :
KLH-conjugated synthetic peptide derived from Natronomonas pharaonis halorhodopsin sequences UniProt: A0A1U7EU03
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Immunofluorescence (IF), Western blot (WB)
Related products :
AS12 1851P NpHR Halorhodopsin blocking peptide
Recommended dilutions :
1 : 200 (IF), 1 : 1000-1 : 20 000 (WB)
Expected apparent MW :
30.9 30 kDa
Confirmed reactivity :
Natronomonas pharaonis
Predicted reactivity :
Natronomonas pharaonis
Not reactive in :
Halobacterium salinarum (HsHR and HsBR)
Additional information :
Immunofluorescence was performed on mouse brain slices (data not shown).
Background :
Halorhodopsin (HR) is a hyperpolarizing light-driven ion pump from the halophilic archaea bacterium Natronomonas pharaonis. HR uses the energy of yellow light (excitation maximum near 580 nm) to mediate primarily chloride but also bromide, iodide, and nitrate import into the cell against their electrochemical gradients.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q3ITX1
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.