product summary
Loading...
company name :
Agrisera
product type :
antibody
product name :
UCP Uncoupling protein
catalog :
AS12 1850
quantity :
100 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS12 1850
Product name :
UCP Uncoupling protein
Immunogen :
KLH-conjugated synthetic peptide derived from known UCP protein sequences, including UCP1 (AT3G54110) and UCP2 (AT5G58970) of Arabidopsis thaliana
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized in PBS pH 7.4
Quantity :
100 µg
Tested applications :
Western blot (WB)
Related products :
antibodies to other plant mitochondrial proteinsPlant and algal protein extraction buffer
Recommended dilutions :
1 : 2000 (WB)
Expected apparent MW :
32 kDa
Confirmed reactivity :
Arabidopsis thaliana, Splanum lycopersicum, Triticum aestivum, Vicia faba (protoplasts)
Predicted reactivity :
Citrus sinensis, Dracunulus vulgaris, Glycine max, Litchi chinensis, Medicago tribuloides, Nicotiana tabacum, Oryza sativa, Populus trichocarpa, Ricinus communis, Saccharum officinarium (sugarcane), Triticum aestivum
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Garmash et al. (2017). Expression profiles of genes for mitochondrial respiratory energy-dissipating systems and antioxidant enzymes in wheat leaves during de-etiolation. J Plant Physiol. 2017 Aug;215:110-121. doi: 10.1016/j.jplph.2017.05.023. Florez-Sarasa et al. (2016). Impaired cyclic electron flow around Photosystem I disturbs high-light respiratory metabolism. Plant Physiol. 2016 Oct 19. pii: pp.01025.2016. Liu et al. (2015). Silencing of mitochondrial uncoupling protein gene aggravates chilling stress by altering mitochondrial respiration and electron transport in tomato. Acta Physiologiae Plantarum November 2015, 37:223. Long et al. (2015). Contributions of photosynthetic and non-photosynthetic cell types to leaf respiration in Vicia faba L. and their responses to growth temperature. Plant Cell Environ. 2015 Apr 1. doi: 10.1111/pce.12544. Grabelnych et al. (2014). Mitochondrial Energy Dissipating Systems (Alternative Oxidase, Uncoupling Proteins, and External NADH Dehydrogenase) Are Involved in Development of Frost Resistance of Winter Wheat Seedlings. ISSN 0006 2979, Biochemistry (Moscow), 2014, Vol. 79, No. 6, pp. 506 519. © Pleiades Publishing, Ltd., 2014.Barreto et al. (2014). Overexpression of UCP1 in tobacco induces mitochondrial biogenesis and amplifies a broad stress response. BMC Plant Biol. 2014 May 28;14(1):144.
Pubmed ID's :
28623839, 27760881, 25828647, 24886177
Background :
UCP (uncoupling protein) is an inner membrane mitochondrial protein that can dissipate the proton gradien before it can be used to provide the energy for oxidative phosphorylation. Synonymes: AtUCP, Uncoupling protein 2.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Peptide used to elicit this antibody is conserved in both isoforms, UCP1 and UCP2 of Arabidopsis thaliana.
TAIR number :
AT3G54110, AT5G58970
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.