product summary
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company name :
Agrisera
product type :
antibody
product name :
V-PPase vacuolar H+-pyrophosphatase
catalog :
AS12 1849
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
cucumber, thale cress
application :
western blot
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
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product information
Product number :
AS12 1849
Product name :
V-PPase vacuolar H+-pyrophosphatase
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-PPase,UniProt P31414, TAIR AT1G15690
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Western blot (WB)
Related products :
collection of antibodies to tonoplast proteins AS09 492 anti-TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 493 anti-TIP1;1, TIP1;2 tonoplast intrinistic protein 1-1, 1-2 (gamma)AS09 494 anti-TIP2;1 (delta) Delta-VM23AS09 510 anti-TIP2;1 tonoplast intrinistic protein 2-1AS09 510 anti-TIP2;2 tonoplast intrinistic protein 2-2AS09 511 anti-TIP2;2 tonoplast intrinistic protein 2-2 (C-terminal)Plant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 8000 (ELISA), 1 : 100 (IL), 1 : 2000 (WB)
Expected apparent MW :
80.8 73 kDa (Arabidopsis thaliana)
Confirmed reactivity :
Arabidopsis thaliana, Cucumis sativus, Dunaliella bardawil, Hordeum vulgare, Nicotiana tabacum, Picea abies
Predicted reactivity :
Oryza sativa, Ricinus communis, Populus trichocarpa, Saccharum officinarum, Vitis vinifera, Zea mays, Vigna radiata
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel.
Selected references :
Migocka et al. (2015). Cucumber Metal Transport Protein CsMTP9 is a plasma membrane H+ -coupled antiporter involved in the Mn2+ and Cd2+ efflux from root cells. Plant J. 2015 Oct 20. doi: 10.1111/tpj.13056. Migocka et al. (2014). Molecular and biochemical properties of two P 1B2 -ATPases, CsHMA3 and CsHMA4, from cucumber. Plant Cell Environ. 2014 Sep 11. doi: 10.1111/pce.12447.
Pubmed ID's :
26485215, 25210955
Background :
V-PPase (pyrophosphate-energized vacuolar membrane proton pump 1), EC=3.6.1.1, acts to establish proton gradient of often higher magnitude than H+-ATPase. Is involved in auxin transport and NaCl and drought tolerance. Alternative names: pyrophosphate-energized inorganic pyrophosphatase 1, H(+)-PPase 1, vacuolar proton pyrophosphatase 1, vacuolar proton pyrophosphatase 3
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibodies will detect target protein in 1 µg of a crude membrane preparation loaded per well. If purified preparations of vacuolar and plasma membranes are used, less than µg load per well should be sufficient.
UniProt number :
P31414
TAIR number :
AT1G15690
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.