product summary
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company name :
Agrisera
product type :
antibody
product name :
Gamma-glutamyl-cysteine
catalog :
AS11 1811
quantity :
2 x100 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
immunocytochemistry
more info or order :
product information
Product number :
AS11 1811
Product name :
Gamma-glutamyl-cysteine
Immunogen :
gamma-glutamyl-cysteine (gamma-EC) linked by glutaraldehyde
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
2 x100 µg
Tested applications :
Immunocytochemistry (ICC), Immunogold (IG)
Related products :
AS06 186 Anti-Gamma-ECS Gamma glutamylcysteine synthase, rabbit antibodyother antibodies to proteins involved in response to heavy metalsPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 50 (ICC), (IG)
Confirmed reactivity :
Arabidopsis thaliana, Cucurbita pepo L. subsp. peop var. styriaca Greb, Nicotiana tabacum cv. samsun
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Immunogold labeling for electron microscopyBlock ultrathin sections (80nm) prepared for immunogold labeling with 2% bovine serum albumine (BSA) in phosphate buffered saline (PBS, pH 7.2). Then treat the sections with the primary antibody (anti-gamma-glutamylcysteine rabbit polyclonal IgG) diluted 1:50 in PBS containing 1% BSA for 2 h at room temperature. After a short rinse in PBS (3 X 5 min), incubate samples with a gold-conjugated secondary antibody (goat anti-rabbit IgG; eg. 10nm) diluted 1:50 in PBS including 1% BSA for 90 min at room temperature. After a short wash in PBS (2 X 5 min), and distilled water (3 X 5 min) observe grids under a transmission electron microscope.
Selected references :
Koffler et al. (2011). Subcellular distribution of glutathione precusors in Arabidopsis thaliana. Journal of Integrative Plant Biology. 53: 930-941.
Pubmed ID's :
22050910
Background :
gamma-glutamyl-cysteine is a precursor of glutathione and is synthesized by linkage of glutamate and cysteine by gamma-glutamylcysteine synthetase. Synonymes:gamma-glutamyl-cysteine, gamma-glutamyl-cysteine. Alternative names: gamma-EC, gamma-Glu-Cys.
Reconstitution :
For reconstitution add 100 µl of sterile water per tube.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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