product summary
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company name :
Agrisera
product type :
antibody
product name :
RPB1 RNA polymerase II subunit B1
catalog :
AS11 1804
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
immunoprecipitation, chromatin immunoprecipitation
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
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product information
Product number :
AS11 1804
Product name :
RPB1 RNA polymerase II subunit B1
Immunogen :
KLH-conjugated synthetic peptide derived from known higher plant polymerase II sequences, including Arabidopsis thaliana P18616, At4g35800
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Immunoprecipitation (IP)
Related products :
AS10 710 anti-H3 histone 3, rabbit antibody collection of antibodies to DN/RNA/cell cyclePlant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1,2 or 5 µg (IP)
Expected apparent MW :
204 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Amygdalus persica, Asparagus officinalis, Brassica rapa, Capsella rubella, Glycine max, Hordeum vulgare, Solanum lycopersicum, Oryza glaberrima, Panicum italicum, Persea americana, Pisum sativum, Ricinus communis, Solanum tuberosum, Sorghum vulgare, Vitis vinifera
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Dolata et al. (2015). NTR1 is required for transcription elongation checkpoints at alternative exons in Arabidopsis. EMBO J. 2015 Jan 7. pii: e201489478.
Pubmed ID's :
25568310
Background :
DNA-directed RNA polymerase II subunit 1 (EC=2.7.7.6) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This enzyme is the central component of the basal RNA polymerase II transcription machinery. Synonymes:DNA-directed RNA polymerase III largest subunit,DNA-directed RNA polymerase II subunit RPB1.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Purified IgG was lyophilized from PBS pH 7.4 + 0.02 % sodium azide. Therefore only distilled water is required for its re-constitution.
UniProt number :
P18616
TAIR number :
AT4G35800
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.