product summary
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company name :
Agrisera
product type :
antibody
product name :
RGL2 RGA-like protein 2 (DELLA protein)
catalog :
AS11 1803
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS11 1803
Product name :
RGL2 RGA-like protein 2 (DELLA protein)
Immunogen :
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana RGL2 protein, UniProt: Q8GXW1, TAIR: At3g03450
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized in PBS pH 7.4
Quantity :
50 µg
Tested applications :
Immunolocalization (IL), Western blot (WB)
Related products :
AS13 2638 anti-GID2 F-BOX protein GID2 (SLEEPY 1), rabbit antibodyAS12 1860 anti-PIF4 Phytochrome interacting factor 4, rabbit antibodyPlant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB), transfected Arabidopsis thaliana protoplasts
Expected apparent MW :
60 kDa
Confirmed reactivity :
Arabidopsis thaliana
Not reactive in :
Triticum sp.
Additional information :
As this protein is expressed in tissue in very low levels, please load at least 50 µg/well.Recommended extraction and denaturation protocol: Arabidopsis thaliana seedlings were ground in liquid nitrogen (100 µl of 2.5 x Laemli for 80-120 mg of homogenized material) and boiled for 2 min. at 95°C in 2,5x Laemmli Buffer (with 60 Mm DTT final concentration) otherwise RGA protein will degrade.
Background :
DELLA protein RGL2 is a probable transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. Involved in the biosynthesis of abscisic acid (ABA), delays flowering and leaf production, reulates floral development, petal, stamen and anther development. Predominantly expressed in germinating seeds, flowers and siliques. Synonymes: GRAS family protein 15, AtGRAS-15, Scarecrow-like protein 19, AtSCL19.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q8GXW1
TAIR number :
AT3G03450
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.