product summary
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company name :
Agrisera
product type :
antibody
product name :
H1 Histone H1
catalog :
AS11 1801
quantity :
50 µg
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, chromatin immunoprecipitation
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
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product information
Product number :
AS11 1801
Product name :
H1 Histone H1
Immunogen :
native H1 protein purified from Nicotiana tabaccum (H1A, H1B H1C,D,E,F)
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Affinity purified serum in PBS, pH 7.4
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
Chromatin Immunoprecipitation (IP) (ChIP), Western blot (WB), Immunocytochemistry (ICC) (ICC)
Related products :
AS10 710 Anti-H3 histone H3, rabbit antibody AS11 1804 Anti-RNA polymerase II subunit B1, rabbit antibodyAS16 3197 Anti-H3 Histone 3 core, monoclonal antibodyAS15 2855 Anti-H3 histone H3, chicken antibodyAS10 710-ALP Anti-H3 Histone H3, ALP-conjugated (40 µg) AS10 710-HRP Anti-H3 Histone H3, HRP-conjugated (40 µg) AS16 3968 Anti-HDT3 Histone deacetylase HDT3. rabbit antibodiescollection of antibodies to DNA/RNA/cell cyclePlant protein extraction buffer
Recommended dilutions :
1 : 100-1 : 500 (ICC), 1 : 5000 (WB)
Expected apparent MW :
15 17 kDa
Confirmed reactivity :
Arabidopsis thaliana, Nicotiana tabacum, Triticum aestivum
Predicted reactivity :
Lathyrus sativus, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Vicia faba
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Selected references :
Benoit et al. (2018). Replication-coupled histone H3.1 deposition determines nucleosome composition and heterochromatin dynamics during Arabidopsis seedling development. New Phytol. 2018 Jun 13. doi: 10.1111/nph.15248. Wollmann et al. (2017). The histone H3 variant H3.3 regulates gene body DNA methylation in Arabidopsis thaliana. Genome Biol. 2017 May 18;18(1):94. doi: 10.1186/s13059-017-1221-3. She and Baroux (2015). Chromatin dynamics in Pollen Mother Cells underpin a common scenario at the somatic-to-reproductive fate transition of both the male and female lineages in Arabidopsis. Front. Plant Sci. doi: 10.3389/fpls.2015.00294. She et al. (2013). Chromatin reprogramming during the somatic to-reproductive cell fate transition in plants. Development Oct;140(19):4008-19. doi: 10.1242/dev.095034. Epub 2013 Sep 4. (Arabidopsis thaliana, immunostaining)
Pubmed ID's :
29897636, 28521766, 24004947
Background :
Histone 1 (H1) is a protein located in nuclei, incorporated into chromatin.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.