product summary
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company name :
Agrisera
product type :
antibody
product name :
FtsH1-11 ATP-dependent zinc metalloprotease FtsH1-11
catalog :
AS11 1789
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 1
product information
Product number :
AS11 1789
Product name :
FtsH1-11 ATP-dependent zinc metalloprotease FtsH1-11
Immunogen :
KLH-conjugated peptide dereived from sequences of all known FtsH isoforms of Arabidopsis thaliana: FtsH1 Q39102 (At1g50250), FtsH2 O80860 (At2g30950), FtsH3 Q84WU8 (At2g29080), FtsH4 O80983 (At2g26140), FtsH5 Q9FH02 (At5g42270), FtsH6 Q1PDW5 (At5g15250), FtsH7 Q9SD67 (At3g47060), FtsH8 Q8W585 (At1g06430), FtsH9 Q9FIM2 (At5g58870), FtsH10 Q8VZI8 (At1g07510), FtsH11 Q9FGM0 (At5g53170) as well as 4 FtsH isoforms of Synechocystis sp. PCC6803NP_440330.1, NP_442160.1, NP_440797.1, NP_440525.1
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Blue Native PAGE (BN-PAGE), Western blot (WB)
Related products :
AS11 1789S FtsH2 positive control/quantitation standardAS16 3930 anti-FtsH1 + FtsH5 ATP-dependent zinc metalloprotease FtsH1 + FtsH5 (chloroplastic)AS16 3929 anti-FtsH2 + FtsH8 ATP-dependent zinc metalloprotease FtsH2 + FtsH8 (chloroplastic)AS07 204 anti-FtsH3 + FtsH10 ATP-dependent zinc metalloprotease FtsH3 + FtsH10 (mitochondrial)AS07 205 anti-FtsH4 ATP-dependent zinc metalloprotease FtsH4 (mitochondrial)AS05 094A anti-FtsH6 ATP-dependent zinc metalloprotease FtsH6 (chloroplastic)AS06 130 anti-FtsH9 ATP-dependent zinc metalloprotease FtsH9 (chloroplastic)AS07 251 anti-FtsH10 ATP-dependent zinc metalloprotease FtsH10 (mitochondrial)Antibodies to other proteins involved in photosynthesisPlant protein extraction bufferSecondary antibodies
Recommended dilutions :
1 : 500-1 : 2000 (WB)
Expected apparent MW :
A. thaliana: FtsH1:76.7, FtsH2: 74, FtsH5: 75.2, FtsH6: 74.5, FtsH7: 87.8, FtsH8: 73, FtsH9:87.8 kDa (chloroplastic); FtsH3: 89.3, FtsH4: 77.2, FtsH10: 89.5 kDa, FtsH11:88.7 kDa (mitochondrial)
Confirmed reactivity :
Acetabularia acetabulum, Arabidopsis thaliana, Bryopsis hypnoides (ulvophytes), Guillardia theta, Hordeum vulgare, Synechocystis sp., Thalassiosira pseudonana, Thalassiosira punctigera, Zea mays
Predicted reactivity :
Diatoms including Phaeodactylum tricornutum, dicots, Chlamydomonas reinhardtii, Monocots
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
For detection on a diatom samples, load of 5-10 µg/well is required as well as usage of enchanced chemiluminescence.The antibody also detects the chloroplast-encoded FtsH from Thalassiosira pseudonana (diatom) along with 3 related isoforms encode in the nuclear genome of Thalassiosira pseudonana.For detection in BlueNative, recommened dilution is 1: 500.
Selected references :
Li et al. (2016). A Hard Day's Night: Diatoms Continue Recycling Photosystem II in the Dark. Front. Mar. Sci., 08 November 2016Tietz et al. (2015). Functional Implications of Photosystem II Crystal Formation in Photosynthetic Membranes. J Biol Chem. 2015 Apr 20. pii: jbc.M114.619841.Campbell et al. (2013). Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana. Photosynth. Res. March 16.
Pubmed ID's :
25897076, 23504483
Background :
FtsH belong to a family of ATP dependent peptidases. Localized in a chloroplast are following isoforms: FTSH1 (synonymes AAA, FTSH, FTSH Protease 1), Ftsh2 (VAR2, VARIEGATED 2), FtsH5 (VAR1, VARIEGATED 1), FtsH6 (FTSH PROTEASE 6), FtsH7, FtsH8. FtsH9.Localized in mitochondria are following isoforms: FtsH3 (FTSH Protease 3), FtsH4, FtsH10, FtsH11.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
Q84WU8 , Q8VZI8
TAIR number :
AT1G50250, AT2G29080
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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