product summary
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company name :
Agrisera
product type :
antibody
product name :
PsbA D1 protein of PSII, N-terminal
catalog :
AS11 1786
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Olimarabidopsis pumila, thale cress
application :
western blot
more info or order :
citations: 1
| Published Application/Species/Sample/Dilution | Reference |
|---|---|
| Fristedt R, Herdean A, Blaby Haas C, Mamedov F, Merchant S, Last R, et al. PHOTOSYSTEM II PROTEIN33, a protein conserved in the plastid lineage, is associated with the chloroplast thylakoid membrane and provides stability to photosystem II supercomplexes in Arabidopsis. Plant Physiol. 2015;167:481-92 pubmed publisher
|
product information
Product number :
AS11 1786
Product name :
PsbA D1 protein of PSII, N-terminal
Immunogen :
KLH-conjugated synthetic peptide derived from N-terminal of available plant, algal and cyanobacterial PsbA sequences, including Arabidopsis thaliana UniProt: A4QJR4, TAIR: AtCg00020 , Oryza sativa P0C434, Populus alba Q14FH6, Physcomitrella patens Q6YXN7, Chlamydomonas reinhardtii P07753, Synechocystis sp. P14660 and many others
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Western blot (WB)
Related products :
AS01 016 anti-PsbA C-terminal chicken antibody AS05 084 anti-PsbA C-terminal rabbit antibody AS11 1786 PsbA D1 protein of PSII, N-terminal, rabbit antibody AS13 2669 PsbA D1 protein of PSII, phosphorylated , rabbit antibody AS05 084PRE PsbA D1 protein of PSII, C-terminal, pre-immune serum AS10 704 anti-PsbA D1 protein of PSII, DE-loop AS01 016S PsbA protein standard for quantitation and positive controlPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000-1 : 10 000 (WB)
Expected apparent MW :
38 28-30 kDa
Confirmed reactivity :
Arabidopsis thaliana, Hordeum vulgare, Chlamydomonas reinhardtii, Chlorella vulgaris, Synechococcus sp. PCC7942, sp. PCC7002, Synechocystis 6803 substrain PCC-M
Predicted reactivity :
Algae (brown and red), Conifers, Cyanobacteria, Diatoms, Glycine max, Medicago truncatula, Nicotiana tabacum, Oryza sativa, Phaseolus vulgaris, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Triticum aestivum, Zea mays, Vitis vinifera
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This antibody will detect the phosphorylated form of D1 as an alternate band to the main band on a high resolution gel.
Selected references :
Liu et al. (2018). Effects of PSII Manganese-Stabilizing Protein Succinylation on Photosynthesis in the Model Cyanobacterium Synechococcus sp. PCC 7002. Plant Cell Physiol. 2018 Jul 1;59(7):1466-1482. doi: 10.1093/pcp/pcy080. Georg et al. (2017). Acclimation of Oxygenic Photosynthesis to Iron Starvation Is Controlled by the sRNA IsaR1. Curr Biol. 2017 May 22;27(10):1425-1436.e7. doi: 10.1016/j.cub.2017.04.010. Perales-Vela et al. (2016). Streptomycin affects the growth and photochemical activity of the alga Chlorella vulgaris. Ecotoxicol Environ Saf. 2016 Oct;132:311-7. doi: 10.1016/j.ecoenv.2016.06.019. Epub 2016 Jun 23.Malnoë et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21. Sook Seok et al. (2013). AtFKBP16-1, a chloroplast lumenal immunophilin, mediates response to photosynthetic stress by regulating PsaL stability. Physiologia Plantarum, DOI: 10.1111/ppl.12116.
Pubmed ID's :
29912468, 28479323, 27344399, 24449688
Background :
The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Peptide target used for antibody production comes from Helix 1 of PSII, lumenal exposed loop. Antibodies are going to recognize the target in a wide range of species.
UniProt number :
A4QJR4
TAIR number :
ATCG00020
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.