product summary
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company name :
Agrisera
product type :
antibody
product name :
ADGP ADP-glucose pyrophosphorylase
catalog :
AS11 1739
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 1
product information
Product number :
AS11 1739
Product name :
ADGP ADP-glucose pyrophosphorylase
Immunogen :
part of Arabidopsis thaliana recombinant ADP-glucose pyrophosphorylase small subunit, TAIR: At5g48300, UniProt: P55228
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
Western blot (WB)
Related products :
other antibodies involved in carbohydrate metabolismPlant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000-1 : 5000 (WB)
Expected apparent MW :
49.4 52 kDa
Confirmed reactivity :
Arabidopsis thaliana, Horderum vulgare, Triticum aestivum, Zea mays
Predicted reactivity :
Chlamydiae, Chlamydomonas reinhardtii, Cyanobacteria, Beta vulgaris, Oryza sativa, Planctomycetes, Proteobacteria, Solanum lycopersicum, Spirochetes, Verrucomicrobia, Vitis vinifera
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Cross reactivity of this antibody to Rubisco has been excluded using 2D gel electrophoresis.
Selected references :
Deng et al. (2018). Comparative Proteome Analysis of Wheat Flag Leaves and Developing Grains Under Water Deficit. Front Plant Sci. 2018 Apr 10;9:425. doi: 10.3389/fpls.2018.00425. eCollection 2018. Yoshida et al. (2018). Thioredoxin-like2/2-Cys peroxiredoxin redox cascade supports oxidative thiol modulation in chloroplasts. Proc Natl Acad Sci U S A. 2018 Aug 28;115(35):E8296-E8304. doi: 10.1073/pnas.1808284115. Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4.
Pubmed ID's :
30104347, 30058028
Background :
ADP-glucose pyrophosphorylase (AGPase) catalyses the first committed step in the synthesis of transient starch in chloroplasts and storage starch in amyloplasts in plants (Tetlow et al., 2004). AGPase in higher plants is composed of two distinct subunits encoded by separate genes, forming an L2S2 heterotetramer. The large subunits (L) are modulators of allosteric activity, while the small subunits (S) are the catalytic subunits (Kim et al., 2007). Synonymes:ADP-glucose pyrophosphorylase, ADP-glucose synthase, AGPase B, Alpha-D-glucose-1-phosphate adenyl transferase
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
P55228 , P55229 , P55231
TAIR number :
AT5G48300
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.