product summary
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company name :
Agrisera
product type :
antibody
product name :
EF1A Elongation factor 1-alpha / EF-1-alpha
catalog :
AS11 1633
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS11 1633
Product name :
EF1A Elongation factor 1-alpha / EF-1-alpha
Immunogen :
full length, recombinant EF1A of Arabidopsis thaliana P13905
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
Related products :
AS10 679 eEF1B-alpha2 elongation factor 1B-alpha 2AS10 678 eEF1B-alpha1 an 2 elongation factor 1B-alpha 1 and 2AS10 677 eEF1B-beta1 and 2 elongation factor 1-beta1 and 1-beta2AS07 265 eEF1b elongation factor eEF1b-beta proteinAS10 676 eEF1B-gamma1 and 2 elongation factor 1-gamma 1 and 2Plant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
49.5 kDa
Confirmed reactivity :
Arabidopsis thaliana, Oryza sativa, Triticum aestivum
Predicted reactivity :
Brassica napus, Picea sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Zea mays
Not reactive in :
Recombinant wheat EF1A
Selected references :
Zhen et al. (2018). 2D-DIGE comparative proteomic analysis of developing wheat grains under high-nitrogen fertilization revealed key differentially accumulated proteins that promote storage protein and starch biosyntheses. Anal Bioanal Chem. 2018 Jul 30. doi: 10.1007/s00216-018-1230-4. Wang et al. (2016). GOLGI TRANSPORT 1B Regulates Protein Export from the Endoplasmic Reticulum in Rice Endosperm Cells. Plant Cell. 2016 Nov;28(11):2850-2865. (Oryza sativa, western blot)
Pubmed ID's :
30058028, 27803308
Background :
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
P13905
TAIR number :
AT1G07940
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.