product summary
Loading...
company name :
Agrisera
product type :
antibody
product name :
DMPO DMPO nitrone adduct
catalog :
AS11 1632
quantity :
100 µl
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgG1 (clone N1664A)
application :
western blot, ELISA, immunocytochemistry, immunoprecipitation
more info or order :
product information
Product number :
AS11 1632
Product name :
DMPO DMPO nitrone adduct
Immunogen :
5,5-dimethyl-2-(8-octanoic acid)-1-pyrrolone-N-oxide conjugated to ovalbumin
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG1 (clone N1664A)
Purity :
Protein G purified
Format :
Liquid
Quantity :
100 µl
Tested applications :
ELISA (ELISA), Immunocytochemistry (ICC), Immuno-spin trapping (IT), Immunoprecipitation (IP), Western blot (WB)
Related products :
collection of antibodies to proteins involved in oxidative stressSecondary antibodies
Recommended dilutions :
1-10 µg/ml (ELISA), 10 µg/ml (ICC), 25 µg (IP), 1-10 µg/ml (WB)
Expected apparent MW :
90 kDa
Confirmed reactivity :
DMPO (species independent)
Predicted reactivity :
DMPO (species independent)
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Important note for immunoprecipitation: do not link antibody to matrix prior to use as this causes large loss of reactivity to DMPO adduct.
Selected references :
Chatterjee et al. (2009). Immuno-spin trapping of a post-translational carboxypeptidase B1 radical formed by a dual role of xanthine oxidase and endothelial nitric oxide synthase in acute septic mice. Free Radic. Med. and Biol. 46:454-461.
Pubmed ID's :
19049863
Background :
DMPO (5,5-dimethly-1-proline N-oxide) is a compound used in spin trapping. It is a method employed in chemistry as an analytical technique for detection and identification of short-lived free radicals. DMPO adducts can be generated with protein and DNA radicals.
Storage :
Store at -20°C for one year. once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody is purified on Protein G and present in TBS pH 7.4 with 0.05 % sodium azide as preservative and 50 % glycerol.DMPO antibody in dilution of 1: 1000 was sufficient to detect the DMPO nitrone adducts of metmyoglobin when loaded at 100 ng/lane by colormetric immunoblot analysis using goat anti-mouse IgG HRP conjugated secondary antibody.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.