RGA DELLA protein RGA | Agrisera AS11 1630 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

RGA DELLA protein RGA

catalog :

AS11 1630

quantity :

50 µg

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 2

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; 1:1000; fig 3	Li K, Yu R, Fan L, Wei N, Chen H, Deng X. DELLA-mediated PIF degradation contributes to coordination of light and gibberellin signalling in Arabidopsis. Nat Commun. 2016;7:11868 pubmed publisher
western blot; thale cress; 1:1000; fig s12	Yang B, Han X, Yin L, Xing M, Xu Z, Xue H. Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016;7:11388 pubmed publisher

product information

Product number :

AS11 1630

Product name :

RGA DELLA protein RGA

Immunogen :

KLH-conjugated peptide chosen from RGA of Arabidopsis thaliana Q9SLH3, At2g01570

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Affinity purified serum in PBS, pH 7.4

Format :

Lyophilized

Quantity :

50 µg

Tested applications :

Western blot (WB)

Related products :

AS11 1631 GAI DELLA protein GAI, rabbit antibody AS06 194 GA3 gibberellic acid, rat antibody AS12 1870 PIF3 Phytochrome interacting factor 3 AS12 1860 PIF4 PHYTOCHROME INTERACTING FACTOR 4 AS11 1802 RGA-like protein DELLA protein RGL1 AS11 1803 RGA-like protein 2 DELLA protein RGL2Plant and algal protein extraction buffer Secondary antibodies

Recommended dilutions :

1 : 1000 (WB)

Expected apparent MW :

64 64 kDa

Confirmed reactivity :

Arabidopsis thaliana

Predicted reactivity :

Arabidopsis thaliana

Not reactive in :

Triticum aestivum

Additional information :

RGA protein is prone to degradation therefore caution has to be taken during protein extraction.

Selected references :

Chahtane et al. (2018). The plant pathogen Pseudomonas aeruginosa triggers a DELLA-dependent seed germination arrest in Arabidopsis. Elife. 2018 Aug 28;7. pii: e37082. doi: 10.7554/eLife.37082. Shahnejat-Bushehri et al. (2016). Arabidopsis NAC transcription factor JUB1 regulates GA/BR metabolism and signalling. NATURE PLANTS 2: Article number: 16013, 2016. Crocco et al. (2015). The transcriptional regulator BBX24 impairs DELLA activity to promote shade avoidance in Arabidopsis thaliana. Nat Commun. 2015 Feb 6;6:6202. doi: 10.1038/ncomms7202. Leone et al. (2014). To grow or defend? Low red : far-red ratios reduce jasmonate sensitivity in Arabidopsis seedlings by promoting DELLA degradation and increasing JAZ10 stability. New Phytol. 2014 Oct;204(2):355-67. doi: 10.1111/nph.12971. Epub 2014 Aug 7.

Pubmed ID's :

30149837, 25656233, 25103816

Background :

DELLA protein RGA is a putative transcriptional regulator that acts as a repressor of the gibberellin (GA) signaling pathway. It is a member of VHIID/DELLA regulatory family and is the most sensitive to GA application, compare to other DELLA proteins.Involved in fruit and flower development. Synonymes: GAI-related sequence, GRAS family protein 10, AtGRAS-10, Repressor on the ga1-3 mutant, Restoration of growth on ammonia protein 1.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

Q9SLH3

TAIR number :

AT2G01570

more info or order :

Agrisera product webpage