product summary
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company name :
Agrisera
product type :
antibody
product name :
HSP18.5 class IV heat shock protein
catalog :
AS11 1628
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
application :
western blot
more info or order :
product information
Product number :
AS11 1628
Product name :
HSP18.5 class IV heat shock protein
Immunogen :
recombinant Arabidopsis thaliana Hsp18.5
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
Related products :
AS08 286 anti-HSP16.6 cytosolic class I heat shock protein 16.6, rabbit antibody for cyanobacterial HSP16.6AS07 254 anti-HSP17.6 cytosolic class I heat shock protein 17.6, rabbit antibodyAS08 284 anti-HSP17.6 cytosolic class I heat shock protein 17.6, hen antibodyAS07 255 anti-HSP17.7 cytosolic class II heat shock protein 17.7, rabbit antibodyAS08 285 anti-HSP21 chloroplastic heat shock protein, rabbit antibodyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
18.5 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Glycne max, Medicago truncatula, Medicago sativa, Pisum sativum, Ricinus communis, Rosa chinensis, Zea mays
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Hsp 18.5 is a low abundancy protein and estimated concentration of this protein in total cell extract is ca. 0.007-0.01 %. Therefore to be able to visualize this protein the load per well needs to be at least 20 ug of heat shocked total protein/well. It is crucial to heat treat the plants at 38ºC for 3 hours at high humidity.Please, note that longer transfer time might result in losing a signal for this protein.
Selected references :
to be added when available.
Background :
HSP18.5 is a small heat shock protein localized in the cytoplasm. The protein belongs to HSP20 family. Synonymes:AtHsp18.5, 18.5 kDa heat shock protein
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
As hsp18.5 is a low abundancy protein, please, make sure that the plants are heated to the right temperature. Normally I heat stress the seedling on a sealed agar plate for 2 hours. This assures that the humidity around the plant is very high. Low humidity can allow the plant to cool down through transpiration. If a plant is in soil you can keep the leaf or even a whole plant over a wet filter paper and seal the plate very well during the treatment. Heat stressing plants in microphage tubes does not work that well.
TAIR number :
AT2G19310
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.