product summary
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company name :
Agrisera
product type :
antibody
product name :
RBR1 Retinoblastoma related protein
catalog :
AS11 1627
quantity :
2 x 50 µl
clonality :
polyclonal
host :
chicken
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot, immunoprecipitation, chromatin immunoprecipitation
more info or order :
product information
Product number :
AS11 1627
Product name :
RBR1 Retinoblastoma related protein
Immunogen :
Recombinant C-terminal fragment consisting of 236 amino acids of Arabidopsis thaliana retinoblastoma protein Q9LKZ3, locus At3g12280
Host :
Chicken
Clonality :
Polyclonal
Purity :
Total IgY
Format :
Liquid in PBS, pH 8.5 with 0.02 % sodium azide
Quantity :
2 x 50 µl
Tested applications :
Chromatin IP (ChIP), Immunoprecipitation (IP), Western blot (WB)
Related products :
Collection of antibodies for plant developmental biologyPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
2µl (IP), 1 : 2000 (WB)
Expected apparent MW :
112 kDa
Confirmed reactivity :
Arabidopsis thaliana, Medicago sativa
Predicted reactivity :
Camelia sinensis, Chenopodium rubrum, Cocos nucifera, Hordeum vulgare, Oryza sativa, Pisum sativum, Populus tremula, Scutelaria baicalensis, Zea mays
Not reactive in :
Chlamydomonas reinhardtii
Additional information :
Antibody is not suitable for immunolocalization.Methanol concentration in a transfer buffer can be considerably reduced or for a better transfer of high MW proteins (even with PVDF membrane).For immunoprecipitation start with 2 µl and titrate it depending upon your experimental conditions. Please note that you work with a total IgY fraction, which means that it will contain between 40-60 µg of total IgY (directed not only against retinoblastoma) therefore all of this IgY needs to be captured by the anti-IgY matrix.As control pre-serum for IP this product can be used, total, pre-immune IgY.
Selected references :
Horvath et al. (2017). Arabidopsis RETINOBLASTOMA RELATED directly regulates DNA damage responses through functions beyond cell cycle control. EMBO J. 2017 May 2;36(9):1261-1278. doi: 10.15252/embj.201694561. Epub 2017 Mar 20. Cheng et al. (2013). Down-regulation of multiple CDK inhibitor ICK/KRP genes up-regulates E2F pathway and increases cell proliferation, organ and seed sizes in Arabidopsis. Plant j. May 7.Ábrahám et al. (2011). Immunodetection of retinoblastoma-related protein and its phosphorylated form in interphase and mitotic alfalfa cells. J Exp Bot 62(6):2155-2168.
Pubmed ID's :
28320736, 23647236, PMC3060694
Background :
The retinoblastoma protein Rb is considered to be a key regulator of G1/S phase transition by blocking S phase entry and cell growth. Plant retinoblastoma-related (RBR) proteins share a homology with the human tumour suppressor retinoblastoma (pRb) protein. RBR protein functions are controlled by phosphorylation and protein-protein interactions. Short name: AtRBR
Storage :
Store at 4°C. Upon arrival please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tubes.
Additional information :
Total IgY concentration is 30.8 mg/ml
UniProt number :
Q9LKZ3
TAIR number :
AT3G12280
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.