product summary
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company name :
Agrisera
product type :
antibody
product name :
eEF1a Elongation factor 1-alpha
catalog :
AS10 934
quantity :
200 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
citations: 1
product information
Product number :
AS10 934
Product name :
eEF1a Elongation factor 1-alpha
Immunogen :
KLH-conjugated synthetic peptide derived from known sequences of eEF1a including Arabidopsis thaliana EF-1-alpha 1P0DH99 AT1G07940 EF-1-alpha 2 Q8W4H7 AT1G07930, EF-1-alpha 3 Q0WL5 AT1G07920, EF-1-alpha 4 Q8GTY0 AT5G6039
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
200 µl
Tested applications :
Western blot (WB)
Related products :
AS11 1633 EF1A elongation factor 1-alpha / EF-1-alpha - antibody made to a recombinant EF1A from Arabidopsis thalianaAS10 679 eEF1B-alpha2 elongation factor 1B-alpha 2AS10 678 eEF1B-alpha1 an 2 elongation factor 1B-alpha 1 and 2AS10 677 eEF1B-beta1 and 2 elongation factor 1-beta1 and 1-beta2AS07 265 eEF1b elongation factor eEF1b-beta proteinAS10 676 eEF1B-gamma1 and 2 elongation factor 1-gamma 1 and 2Plant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Expected apparent MW :
49.5 kDa
Confirmed reactivity :
Arabidopsis thaliana, Hordeum vulgare, Olea europaea, Physcomitrella patens, Solanum tuberosum, Zea mays
Predicted reactivity :
Daucus carota, Euglena gracilis (and unicellular flagellate protists) , Glycine max, Lilium longiflorum, Malus domestica, Nicotiana tabacum, Oryza sativa, Pisum sativum, Solanum lycopersicum, Triticum aestivum, Vicia faba
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
This product can be sold containing ProClin if requested.Following protein resolubilization with urea/thiourea/CHAPS signal strength of recognized band is decreased. Recommended protein extraction conditions are without urea.
Selected references :
Foley et al. (2017). A Global View of RNA-Protein Interactions Identifies Post-transcriptional Regulators of Root Hair Cell Fate.Dev Cell. 2017 Apr 24;41(2):204-220.e5. doi: 10.1016/j.devcel.2017.03.018.
Pubmed ID's :
28441533
Background :
EF1A (elongation factor 1-alpha) belongs to the GTP-binding elongation factor family and is localized in the cytoplasm. It is an essential enzyme in elongation phase of protein synthesis. There are four genes encoding EF1A in Arabidopsis, sequences of genes 1,2 and 3 are identical. Synonymes:eEF-1A.
Reconstitution :
For reconstitution add 200 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
AT1G07920 , AT1G07930 , AT1G07940 , AT5G6039
TAIR number :
AT1G07920, AT1G07930, AT1G07940, AT5G6039
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.