product summary
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company name :
Agrisera
product type :
antibody
product name :
Amyloid beta oligomer-specific monoclonal antibody (OMAB)
catalog :
AS10 932
quantity :
50 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgM
reactivity :
human
application :
ELISA, immunohistochemistry
more info or order :
citations: 1
product information
Product number :
AS10 932
Product name :
Amyloid beta oligomer-specific monoclonal antibody (OMAB)
Immunogen :
partly aggregated, recombinant peptide corresponding to the human Abeta (1-40/42). Amino acid sequence: D-A-E-F-R-H-D-S-G-Y-E-V-H-H-Q-K-L-V-F-F-A-E-D-V-G-S-N-K-G-A-I-I-G-L-M-V-G-G-V-V. The epitope is 3-8. Molecular weight of immunogen is 4.5 kDa.
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgM
Purity :
Affinity purified
Format :
Lyophilized
Quantity :
50 µg
Tested applications :
ELISA (ELISA), Immunohistochemistry (IHC)
Related products :
AS13 2716 mAB-M human Abeta protein (3-10) region, oligomer-specific, mouse monoclonal antibodyAS13 2715 mAB-O human Abeta protein (3-10) region, oligomer specific, mouse monoclonal antibodyAgrisera matching secondary antibody: Goat anti-mouse IgM (µ chain), HRP conjugated, min. cross-reactivity to human IgG/serum, AS10 969Secondary antibodies
Recommended dilutions :
Coating antibody at 2 µg/ml (ELISA), 1 : 500 (IHC)
Expected apparent MW :
4.5 kDa
Confirmed reactivity :
Human Abeta oligomers only
Predicted reactivity :
Rat
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
OMAB antibody is a versatile tool within research of Alzheimer’s disease. A sandwhich ELISA illustrates its potential regarding its high selectivity towards Abeta oligomers.
Selected references :
Kumar et al. (2018). Peptidomimetic-Based Multidomain Targeting Offers Critical Evaluation of Abeta Structure and Toxic Function. J Am Chem Soc. 2018 May 30;140(21):6562-6574. doi: 10.1021/jacs.7b13401. Kumar et al. (2017). Foldamer-Mediated Structural Rearrangement Attenuates Abeta Oligomerization and Cytotoxicity. J Am Chem Soc. 2017 Nov 29;139(47):17098-17108. doi: 10.1021/jacs.7b08259. Zhao et al. (2016). Antiamyloidogenic Activity of Abeta42-Binding Peptoid in Modulating Amyloid Oligomerization. Small. 2016 Oct 7. doi: 10.1002/smll.201602857.Richman et al. (2013). In Vitro and Mechanistic Studies of an Anti-Amyloidogenic Self-Assembled Cyclic D,L-#-Peptide Architecture. J. Americal Chemical Societ, Jan 19.Lindhagen-Persson et al. (2010). Amyloid-beta Oligomer Specificity Mediated by the IgM Isotype – Implications for a Specific Protective Mechanism Exerted by Endogenous Auto-Antibodies. PLoS ONE.
Pubmed ID's :
29648815, 29058422, 27714968, 21085663
Background :
Soluble oligomeric assemblies of the Amyloid-beta peptide are today anticipated to be the direct cause regarding the Alzheimer pathology. As a consequence, oligomeric Abeta-assemblies constitute a very interesting therapeutic target. Identification of Abeta-oligomers is however, technically challenging due to there labile nature and low abundance. Abeta oligomer-specific OMAB antibody is based on the IgM isotype and represents a new concept of Abeta-oligomer binders using a combination of high avidity and very low monovalent affinity. This combination creates a selectivity of the antibody towards the oligomeric fraction and minimizes reactivity towards monomeric species.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at 4°C. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
OMAB antibody has been purified by by ion-exchange chromatography and is supplied in PBS without any additives as carrier proteins or sodium azide.Binding of OMAB antibody and Abeta oligomers at RT takes about 15 min.Fibrils are inaccessible for OMAB antibodies therefore if a discrimination between fibrils and oligomers is to be achieved, dot blot can be used. Start with antigen concentration of 500 ng/dot followed by 2X dilution steps. Blocking: non-fat milk and washes with 0.3 % Tween 20 in TBS pH 7.4.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.