PGRL1 PGR5-like protein 1A (chloroplastic) | Agrisera AS10 725 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Agrisera

product type :

antibody

product name :

PGRL1 PGR5-like protein 1A (chloroplastic)

catalog :

AS10 725

quantity :

100 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

product information

Product number :

AS10 725

Product name :

PGRL1 PGR5-like protein 1A (chloroplastic)

Immunogen :

the N-terminal loop (aa 61 to 200; N-PGRL1) of PGRL1A (Q8H112, At4g22890). The N-PGRL1 protein was purified by virtue of its His-Tag using Ni-NTA Agarose (QIAGEN).

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

100 µl

Tested applications :

Western blot (WB)

Related products :

collection of antibodies to photosynthetic proteinsPlant and algal protein extraction buffer Secondary antibodies

Recommended dilutions :

1 : 3000 (WB)

Expected apparent MW :

29 29 kDa (Arabidopsis thaliana, Spinacia oleracea)

Confirmed reactivity :

Arabidopsis thaliana, Spinacia oleracea

Predicted reactivity :

Cucumis sativus, Glycine max, Ricinus communis, Vitis vinifera, Populus trichocarpa, Oryza sativa, Zea mays, Zosteria marina

Not reactive in :

Chlamydomonas reinhardtii, Nicotiana tabacum, Physcomitrella patens

Additional information :

In Arabidopsis thaliana samples cross-reactivity in the region of 55-60 kDa can be detected.This product can be sold containing ProClin if requested

Selected references :

Nikkanen et al. (2018). Regulation of chloroplast NADH dehydrogenase-like complex by NADPH-dependent thioredoxin system. CSH, BioRixiv. doi.org/10.1101/261560. Lintala et al. (2013). Arabidopsis tic62 trol mutant lacking thylakoid bound ferredoxin-NADP+ oxidoreductase shows distinct metabolic phenotype. Mol Plant Sep 16. Endow and Inoue (2013).Stablecomplexformation ofthylakoidalprocessingpeptidase andPGRL1. FEBS Lett. May 31st.

Pubmed ID's :

24043709, 23727204

Background :

PGRL1 is a transmembrane protein present in thylakoids of higher plants and algae. Arabidopsis plants lacking PGRL1 show perturbation of cyclic electron transport (CEF) around photosystem I (PSI), similar to PGR5-deficient plants. PGRL1 has been shown to interact physically with PGR5 and associate with PSI (DalCorso et al., 2008). In Chlamydomonas reinhardtii, PGRL1 is part of a protein supercomplex, composed of PSI with its own light-harvesting complex (LHCI), the photosystem II light-harvesting complex (LHCII), the cytochrome b6/f complex (Cyt b6f), ferredoxin (Fd)-NADPH oxidoreductase (FNR), responsible of the energy balance of the two photosystems and of the switch between thylakoid linear and cyclic electron transport (Iwai et al., 2010). Synonymes:

Reconstitution :

For reconstitution add 100 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

UniProt number :

828388

TAIR number :

AT4G22890