product summary
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company name :
Agrisera
product type :
antibody
product name :
PHOT1 Phototropin-1
catalog :
AS10 720
quantity :
100 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
thale cress
application :
western blot
more info or order :
product information
Product number :
AS10 720
Product name :
PHOT1 Phototropin-1
Immunogen :
KLH-conjugated synthetic peptide derived from known Arabidopsis thaliana PHOT1 O48963, At3g45780
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Western blot (WB)
Related products :
AS10 721 PHOT2 phototropin-2Plant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 5000 (WB)
Expected apparent MW :
111 132 kDa
Confirmed reactivity :
Arabidopsis thaliana
Predicted reactivity :
Arabidopsis thaliana
Not reactive in :
Oryza sativa
Additional information :
Information about phot1 mutant, first named nph1: Liscum &. Briggs (1995). Mutations in the NPH1 Locus of Arabidopsis Disrupt the Perception of Phototropic Stimuli. The Plant Cell, Vol. 7, 473-485.Huala et al. (1997). Arabidopsis NPH1: A Protein Kinase with a Putative Redox-Sensing Domain. Science 19: Vol. 278 no. 5346 pp. 2120-2123.Lehmann et al. (2011). Transitions of gene expression induced by short-term blue light. Plant Biology Volume 13, Issue 2, pages 349–361. Seeds of this mutant are available at uNASC.
Selected references :
Eckstein et al. (2015). Auxin and chloroplast movements. Physiol Plant. 2015 Oct 15. doi: 10.1111/ppl.12396. Åabuz et al. (2015). The impact of temperature on blue light induced chloroplast movements in Arabidopsis thaliana. Plant Science, doi:10.1016/j.plantsci.2015.07.013.
Pubmed ID's :
26467664
Background :
PHOT1 phototropin-1 is a blue-light photoreceptor which containst light activated serine-threonine kinase domain. Is required for stomatal opening, chloroplast movements, leaf flattening and phototropism. Undergoes blue-light-dependent autophosphorylation. Alternative names: NPH1, RPT1, JK224, Non-phototropic hypocotyl protein 1, Root phototropism protein 1
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
This product can be sold containing ProClin if requested.
UniProt number :
O48963
TAIR number :
AT3G45780
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.