product summary
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company name :
Agrisera
product type :
antibody
product name :
FtsZ Procaryotic cell division GTPase
catalog :
AS10 715
quantity :
50 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Escherichia coli str. K-12 substr. MG1655, Escherichia coli
application :
western blot, immunocytochemistry
more info or order :
citations: 1
Published Application/Species/Sample/DilutionReference
  • immunocytochemistry; Escherichia coli; 1:200; fig 1
Vedyaykin A, Vishnyakov I, Polinovskaya V, Khodorkovskii M, Sabantsev A. New insights into FtsZ rearrangements during the cell division of Escherichia coli from single-molecule localization microscopy of fixed cells. Microbiologyopen. 2016;5:378-86 pubmed publisher
product information
Product number :
AS10 715
Product name :
FtsZ Procaryotic cell division GTPase
Immunogen :
KLH-conjugated synthetic peptide derived from known bacterial sequences of FtsZ including E.coli P0A9A6
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
50 µl
Tested applications :
immunofluorescence (IF), Western blot (WB)
Related products :
AS07 217 FtsZ procaryotic cell division GTPase, cyanobacterial, rabbit antibodyAS09 413 anti-FtsZ1 and 2 plant cell division protein ftsZ1 and ftsZ2 , rabbit antibody AS13 2651 anti-FtsZ2 plant cell division protein ftsZ2, rabbit antibodySecondary antibodies
Recommended dilutions :
1 : 100-1 : 200 (IF), 1 : 1000 (WB)
Expected apparent MW :
40 42 kDa
Confirmed reactivity :
E coli DH5a, Shigella flexneri
Predicted reactivity :
Candidatus sp., Citrobacter sp. 30_2, Dickeya sp., Enterobacter sp., Klebsiella pneumoniae subsp. pneumoniae MGH, Salmonella sp., Shigella sonnei Ss046, Vibrio sp., Yersinia pestis D182038
Not reactive in :
B. subtilis, Listera sp., Neisseria meningitidis, Pseudomonas aeruginosa, Staphylococcus aureus (strain MRSA252), cyanobacteria
Selected references :
Mückl et al. (2018). Filamentation and restoration of normal growth in Escherichia coli using a combined CRISPRi sgRNA/antisense RNA approach. PLoS One. 2018 Sep 11;13(9):e0198058. doi: 10.1371/journal.pone.0198058. eCollection 2018. Pende et al. (2014). Size-independent symmetric division in extraordinarily long cells. Nat Commun. 2014 Sep 15;5:4803. doi: 10.1038/ncomms5803. Söderström et al. (2014). Disassembly of the divisome in Escherichia coli: Evidence that FtsZ dissociates before compartmentalisation. Mol Microbiol. 2014 Feb 7. doi: 10.1111/mmi.12534. (western blot and immunofluorescence)
Pubmed ID's :
30204770, 25221974, 24506818
Background :
FtsZ (cell division GTPase) is a well characterized protein of the bacterial cell division apparatus. This protein accumulates early in dividing cells, and has a crucial role during septum formation in most bacteria. It has also been accepted as the bacterial cytoskeletal counterpart to eukaryotic microtubules. Synonymes: sifB, SulB.
Reconstitution :
For reconstitution add 50 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
UniProt number :
P0A9A6
more info or order :
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.