product summary
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company name :
Agrisera
product type :
antibody
product name :
AMY Alpha-amylase
catalog :
AS10 712
quantity :
100 µl
clonality :
polyclonal
host :
rabbit
conjugate :
nonconjugated
reactivity :
Japanese rice
application :
western blot
more info or order :
product information
Product number :
AS10 712
Product name :
AMY Alpha-amylase
Immunogen :
KLH-conjugated synthetic peptide derived from known Oryza sativa P17654
Host :
Rabbit
Clonality :
Polyclonal
Purity :
Serum
Format :
Lyophilized
Quantity :
100 µl
Tested applications :
Western blot (WB)
Related products :
AS10 716 Ramy 3D Alpha-amylase isozyme 3DPlant protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 5000 (WB)
Expected apparent MW :
43-48 kDa (depending upon the isoform)
Confirmed reactivity :
Oryza sativa, Panicum virgatum
Predicted reactivity :
Cereals, Kalanchoe laxiflora
Not reactive in :
Pinus strobus
Selected references :
Ye et al. (2018). Natural variation in the promoter of rice calcineurin B-like protein10 (OsCBL10) affects flooding tolerance during seed germination among rice subspecies. Plant J. 2018 May;94(4):612-625. doi: 10.1111/tpj.13881.
Pubmed ID's :
29495079
Background :
alpha-Amylases are hydrolytic enzymes responsible for the mobilization of the starch into metabolizable sugars. This process provides the energy for the growth of roots and shoots and is crucial during germination of cereal seeds.These enzymes are coded by a multigene family and even thought other amylolytic enzyme participate in the process of starch breakdown, the contribution of alpha-amylase is the prerequisite for the initiation of this process.
Reconstitution :
For reconstitution add 100 µl of sterile water.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Antibody will detect all alpha amylase isoforms from rice, barley and other cereals
UniProt number :
P17654
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.