H3 Histone H3 (rabbit antibody) (nuclear marker) | Agrisera AS10 710 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

H3 Histone H3 (rabbit antibody) (nuclear marker)

catalog :

AS10 710

quantity :

50 µl

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, thale cress

application :

western blot, immunocytochemistry

more info or order :

Agrisera product webpage

citations: 6

Published Application/Species/Sample/Dilution	Reference
western blot; thale cress; 1:1500; fig s1	Zhang H, Cui F, Wu Y, Lou L, Liu L, Tian M, et al. The RING finger ubiquitin E3 ligase SDIR1 targets SDIR1-INTERACTING PROTEIN1 for degradation to modulate the salt stress response and ABA signaling in Arabidopsis. Plant Cell. 2015;27:214-27 pubmed publisher
	Cejas R, Lorenz V, Garay Y, Irazoqui F. Biosynthesis of O-N-acetylgalactosamine glycans in the human cell nucleus. J Biol Chem. 2019;294:2997-3011 pubmed publisher
	Wang X, Wang Y, Dou Y, Chen L, Wang J, Jiang N, et al. Degradation of unmethylated miRNA/miRNA*s by a DEDDy-type 3' to 5' exoribonuclease Atrimmer 2 in Arabidopsis. Proc Natl Acad Sci U S A. 2018;115:E6659-E6667 pubmed publisher
	Zhang Z, Guo X, Ge C, Ma Z, Jiang M, Li T, et al. KETCH1 imports HYL1 to nucleus for miRNA biogenesis in Arabidopsis. Proc Natl Acad Sci U S A. 2017;114:4011-4016 pubmed publisher
	Yang B, Han X, Yin L, Xing M, Xu Z, Xue H. Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling. Nat Commun. 2016;7:11388 pubmed publisher
	Wobbe L, Nixon P. The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii. Nucleic Acids Res. 2013;41:6553-67 pubmed publisher

product information

Product number :

AS10 710

Product name :

H3 Histone H3 (rabbit antibody) (nuclear marker)

Immunogen :

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030, At4g40040, At5g10980), H3.2 P59226 (At1g09200, At3g27360, At5g10390, At5g10400, At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

ChIp-qPCR (ChIp-qPCR), Immunocytochemistry (ICC), Western blot (WB)

Related products :

AS11 1804 Anti-RNA polymerase II subunit B1, rabbit antibodyAS16 3197 Anti-H3 Histone 3 core, monoclonal antibodyAS15 2855 Anti-H3 histone H3, chicken antibodyAS10 710-ALP Anti-H3 Histone H3, ALP-conjugated (40 µg) AS10 710-HRP Anti-H3 Histone H3, HRP-conjugated (40 µg) AS10 710PRE H3 histone H3, pre-immune serum (control for immunolocalization)collection of antibodies to DNA/RNA/cell cyclePlant and algal protein extraction buffer

Recommended dilutions :

1 : 100-1 : 500 (ICC), 2 µl of antibody/500 µl solution (ChIp-qPCR), 1 : 5000 (WB)

Expected apparent MW :

15 17 kDa

Confirmed reactivity :

Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Cicer arietinum L., Chlamydomonas acidophila, Chlamydomonas reinhardtii, Cucumis sativus L cv Suyo, human, Nicotiana benthamiana, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays

Predicted reactivity :

Brachypodium distachyon, Brassica napus, Emiliania huxleyi, Hordeum vulgare, Nicotiana tabacum, Malus domestica, Medicago sativa, Triticum aestivum, Pinus pinaster, Pisum sativum, Oryza sativa, Zea mays, Vitis vinifera, Volvox sp.

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

Selected references :

Barua et al. (2018). Dehydration-responsive nuclear proteome landscape of chickpea (Cicer arietinum L.) reveals phosphorylation-mediated regulation of stress response. Plant Cell Environ. 2018 May 10. doi: 10.1111/pce.13334. Lai et al. (2018). Salicylic acid-independent role of NPR1 is required for protection from proteotoxic stress in the plant endoplasmic reticulum. Proc Natl Acad Sci U S A. 2018 May 29;115(22):E5203-E5212. doi: 10.1073/pnas.1802254115. Wang et al. (2018). Degradation of unmethylated miRNA/miRNA*s by a DEDDy-type 3' to 5' exoribonuclease Atrimmer 2 in Arabidopsis. Proc Natl Acad Sci U S A. 2018 Jul 10;115(28):E6659-E6667. doi: 10.1073/pnas.1721917115. Hartmann et al. (2018). Subcellular Compartmentation of Alternatively Spliced Transcripts Defines SERINE/ARGININE-RICH PROTEIN30 Expression. Plant Physiol. 2018 Apr;176(4):2886-2903. doi: 10.1104/pp.17.01260. Duan et al. (2017). A Lipid-Anchored NAC Transcription Factor Is Translocated into the Nucleus and Activates Glyoxalase I Expression during Drought Stress. Plant Cell. 2017 Jul;29(7):1748-1772. doi: 10.1105/tpc.17.00044. (Nicotiana benthamiana)Rihan et al. (2017). An analysis of the development of cauliflower seed as a model to improve the molecular mechanism of abiotic stress tolerance in cauliflower artificial seeds. Plant Physiol Biochem. 2017 Jul;116:91-105. doi: 10.1016/j.plaphy.2017.05.011. Shin et al. (2017). The metabolic sensor AKIN10 modulates the Arabidopsis circadian clock in a light-dependent manner. Plant Cell Environ. 2017 Jan 5. doi: 10.1111/pce.12903. Correa-Galvis et al. (2016). Photosystem II Subunit PsbS Is Involved in the Induction of LHCSR Protein-dependent Energy Dissipation in Chlamydomonas reinhardtii. J Biol Chem. 2016 Aug 12;291(33):17478-87. doi: 10.1074/jbc.M116.737312.Castellano et al. (2016). A pathogenic long noncoding RNA redesigns the epigenetic landscape of the infected cells by subverting host Histone Deacetylase 6 activity. New Phytol. 2016 Sep;211(4):1311-22. doi: 10.1111/nph.14001. Epub 2016 May 12.Ghandi et al. (2016). Tomato yellow leaf curl virus infection mitigates the heat stress response of plants grown at high temperature. Sci Rep. 2016 Jan 21;6:19715. doi: 10.1038/srep19715.Gorovits et al. (2016). Tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates. Virus Res. 2016 Feb 2;213:304-13. doi: 10.1016/j.virusres.2015.11.020. Epub 2015 Dec 1

Pubmed ID's :

29749054, 29760094, 29941559, 29496883, 28684428, 28551420, 28054361, 27358399, 27174164, 26792235, 26654789

Background :

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

UniProt number :

P59169 , P59226 , Q9FXI7

TAIR number :

AT4G40030

more info or order :

Agrisera product webpage