H3 histone H3 (100 µl) | Agrisera AS10 710-100 product information

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product summary

company name :

Agrisera

product type :

antibody

product name :

H3 histone H3 (100 µl)

catalog :

AS10 710-100

quantity :

2 x 50 µl

price :

392.00 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot, immunocytochemistry

product information

Product number :

AS10 710-100

Product name :

H3 histone H3 (100 µl)

Price (EUR) :

392.00 USD

Immunogen :

KLH-conjugated synthetic peptide derived from known H3 sequences, inluding Arabidopsis thaliana H3.3 P59169 (At4g40030,At4g40040,At5g10980), H3.2 P59226(At1g09200,At3g27360,At5g10390,At5g10400,At5g65360), H3-like 2 Q9FXI7 (At1g19890)

Host :

rabbit

Clonality :

polyclonal

Purity :

serum

Format :

lyophilized

Quantity :

2 x 50 µl

Tested applications :

western blot (WB), immunocytochemistry (ICC)

Related products :

AS11 1804 RNA polymerase II subunit B1, rabbit antibodyAS10 710PRE H3 histone H3, pre-immune serumcollection of antibodies to DNA/RNA/cell cycle

Recommended dilutions :

1 : 5000 with standard ECL (WB), 1: 100 - 1: 500 (ICC)

Expected apparent MW :

15 17 kDa

Confirmed reactivity :

Arabidopsis thaliana, Brassica oleracea, Capsicum annuum, Chlamydomonas acidophila, Chlamydomonas reinhardtii, Physcomitrella patens, Salicornia europaea, Solanum lycopersicum, Solanum sogarandinum, Solanum tuberosum, Vicia faba, Zea mays

Predicted reactivity :

dicots including: Vitis vinifera, monocots: Hordeum vulgare, Oryza sativa, Zea mays. Nicotiana tabacum, Medicago sativa, Triticum aestivum, Pisum sativum, Brassica napus. trees: Pinus pinaster, algae: Volvox sp.

Not reactive in :

no confirmed exceptions from predicted reactivity known in the moment

Storage :

store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Reconstitution :

for reconstitution add 50 µl, of sterile water to each tube

Additional information :

Protocol for isolation of cytosolic and nuclear fractions can be found here.Specific fluorescence in ICC has been observed for interphase nuclei as well as around centromer region (where Ser10 of histone H3 is phosphorylated) in mitotic chromosomes.

Selected references :

Spijkerman et al. (2014). CO2 acquisition in Chlamydomonas acidophila is influenced mainly by CO2, not phosphorus, availability. Photosynth Res. 2014 Sep;121(2-3):213-21. doi: 10.1007/s11120-014-0016-6. Epub 2014 Jun 7. Rihan et al. (2014). The effect of molybdenum on the molecular control of cold tolerance in cauliflower (Brassica oleracea var. botrytis) artificial seeds. Plant Cell, Tissue and Organ Culture (PCTOC) April 2014 Szabala et al. (2014). Accumulation of acidic SK3 dehydrins in phloem cells of cold- and drought-stressed plants of the Solanaceae. Planta, Jan 7. Wobbe and Nixon (2013). The mTERF protein MOC1 terminates mitochondrial DNA transcription in the unicellular green alga Chlamydomonas reinhardtii. Nucleic Acids Res. May 6.

Background :

Histone 3 (H3) located in nuclei, incorporated into chromatin. Present in nucleosome together with H2A, H2B and H4.

Special application note :

cellular [compartment marker] of nucleoplasm, loading control antibody for Chlamydomonas reinhardtii

UniProt number :

P59169, P59226, Q9FXI7

TAIR number :

AT4G40030