product summary
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company name :
Agrisera
product type :
antibody
product name :
8-Hydroxyguanosine DNA/RNA oxidative damage
catalog :
AS10 708-100
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgG2A (clone 15A3)
application :
ELISA
more info or order :
product information
Product number :
AS10 708-100
Product name :
8-Hydroxyguanosine DNA/RNA oxidative damage
Immunogen :
8-hydroxy-guanosine-BSA and – casein conjugates
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG2A (clone 15A3)
Purity :
Protein G purified
Format :
Liquid
Quantity :
100 µg
Tested applications :
ELISA (ELISA), Immunoaffinity chromatography (IAP), Immunohistochemistry on frozen tissue and paraffin-embedded (IHC-Fr-P)
Related products :
AS10 708-25 hydroxy guanosine monoclonal antibody, 25 µgSecondary antibodies
Recommended dilutions :
The optimal working dilution should be determined by the investigator.
Confirmed reactivity :
Recognizes markers of oxidative damage to DNA (8-hydroxy-2’-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
Protocol for immunostaining using this antibody can be found here.
Selected references :
Haigh and Drew (2015). Cavitation during the protein misfolding cyclic amplification (PMCA) method - The trigger for de novo prion generation? Biochem Biophys Res Commun. 2015 Apr 17. pii: S0006-291X(15)00726-3. doi: 10.1016/j.bbrc.2015.04.048. Poborilova et al. (2015). DNA hypomethylation concomitant with the overproduction of ROS induced by naphthoquinone juglone on tobacco BY-2 suspension cells. Environmental and Experimental Botany, Volume 113, May 2015, Pages 28–39.
Pubmed ID's :
25892519
Background :
Oxidative derivate of guanosine is called 8-Hydroxyguanosine (8OHdG) and is used as a popular biomarker of oxidative stress.
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 0.65 mg/ml.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.
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