product summary
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company name :
Agrisera
product type :
antibody
product name :
Acetylated lysine (25 µl)
catalog :
AS10 707-25
quantity :
25 µl
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
antigen modification :
acetylated
clone name :
IgG1 (clone 7F8)
application :
western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
Agrisera product webpage
product information
Product number :
AS10 707-25
Product name :
Acetylated lysine (25 µl)
Immunogen :
acetylated KLH
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG1 (clone 7F8)
Purity :
Protein G purified
Format :
Liquid
Quantity :
25 µl
Tested applications :
ELISA (ELISA), Immunocytochmistry/Immunofluorescence (ICC/IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western blot (WB)
Related products :
AS15 3018 anti-Acetylated Lysine, rabbit antibodies AS10 707-100 anti-Acetylated lysine (100 µl), mouse antibody AS15 3019 anti-Acetylated Lysine, APL conjugated, rabbit antibodyAS15 3020 anti-Acetylated Lysine, FITC conjugated, rabbit antibodycollection of antibodies to epigeneticsSecondary antibodies
Recommended dilutions :
1 : 100 (IHC), 1 : 1000 (WB)
Confirmed reactivity :
Bovine, avian
Predicted reactivity :
Higher plants
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
1 µg of this antibody is sufficient to detect acetylated chicken erythrocyte histones (sodium butyrate-treated) using 20 µg total protein and ECL detection system
Selected references :
Vigushin & Coombes (2004). Targeted histone deacetylase inhibition for cancer therapy. Curr. Cancer Drug Targets 4: 205-218.
Pubmed ID's :
15032670
Background :
Post-translational modifications of proteins play critical roles in the regulation and function of many known biological processes. Proteins can be post-translationally modified in many different ways, and a common posttranscriptional modification of Lysine involves acetylation (1). The conserved amino-terminal domains of the four core histones (H2A, H2B, H3 and H4) contain lysines that are acetylated by histone acetyltransferases (HATs) and deacetylated by histone deacetylases (HDACs) (2). Protein posttranslational reversible lysine Nepsilon-acetylation and deacetylation have been recognized as an emerging intracellular signaling mechanism that plays critical roles in regulating gene transcription, cell-cycle progression, apoptosis, DNA repair, and cytoskeletal organization (3).
Storage :
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Protein G purified IgG2B in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/mlantibody detects Proteins containing acetylated lysine residues in ELISA and WBs. Does not detect non-acetylated lysine residues.
more info or order :
Agrisera product webpage
company information
Agrisera
Box 57
911 21 Vannas
info@agrisera.com
http://www.agrisera.com
46 935-330000
headquarters: Sweden
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.