product summary
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company name :
Agrisera
product type :
antibody
product name :
P-Tyr Phosphotyrosine
catalog :
AS10 705
quantity :
100 µg
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
IgG1 (clone G104)
application :
western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation
more info or order :
product information
Product number :
AS10 705
Product name :
P-Tyr Phosphotyrosine
Immunogen :
Phosphotyrosine, alanine and glyceine in a 1:1:1 ratio polymerized in the presence of keyhole limpet hemocyanin KLH with 1-ethyl-3-(3’-dimentrylaminopropyl) carbodiimide
Host :
Mouse
Clonality :
Monoclonal
Clone number :
IgG1 (clone G104)
Purity :
Protein G purified
Format :
Liquid
Quantity :
100 µg
Tested applications :
Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC), Western blot (WB)
Related products :
Plant and algal protein extraction buffer Secondary antibodies
Recommended dilutions :
1 : 1000 (WB)
Confirmed reactivity :
Antibody reacts with phosphotyrosine and detects the presence of phosphotyrosine in proteins of both unstimulated and stimulated cell lysated. Does not cross react with phosphoserine or phosphothreonine.
Not reactive in :
No confirmed exceptions from predicted reactivity are currently known.
Additional information :
1 µg/ml of this antibody is sufficient for detection of phosphorylated tyrosine residues in 10 µg of rat tissue lysate by colorimetric immunoblot analysis.
Selected references :
Garton et al. (1996). Identification of p130(cas) as a substrate for the cytosolic protein tyrosine phosphatase PTP-PEST. Mol and Cell Bio 11:6408-6418.(IP):Tiganis et al. (1999). The protein-tyrosine phosphatase TCPTP regulates epidermal growth factor receptor-mediated and phosphatidylinositol 3-kinase-dependent signaling. J Biol Chem 39: 27768-27775.(IF): Garton & Tonks (1999). Regulation of fibroblast motility by the protein tyrosine phosphatase PTP-PEST. J Biol Chem 6:3811-3818.
Pubmed ID's :
8887669, 10488121, 9920935
Background :
Tyrosine phosphorylation is considered to be one of the key steps in signal transduction and regulation of enzymatic activity. Phosphotyrosine antibodies are helpful in facilitating the identification of tyrosine kinase substrates.
Storage :
Store at -20°C for one year; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Additional information :
Protein G purified IgG1 in PBS, pH 7.4 with 0.09 % sodium azide and 50 % glycerol at concentration 1 mg/ml.
more info or order :
company information
Agrisera
Agrisera is a Swedish company specializing in polyclonal and monoclonal antibody production, offering primary and secondary antibodies off-the-shelf. Agrisera offers an extensive list of antibodies suitable for detection of plant and algal proteins in a wide range of research areas and applications. They are reactive in thousands of plant and algal species and cited in thousands of scientific articles. Agrisera was awarded as the Plant Science Antibody Supplier of the Year by CiteAB for the company with the most antibody citations for research related to plant science during 2018.