PsbA D1 protein of PSII, DE-loop | Agrisera AS10 704 product information

product summary

company name :

Agrisera

product type :

antibody

product name :

PsbA D1 protein of PSII, DE-loop

catalog :

AS10 704

quantity :

50 µl

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

reactivity :

thale cress

application :

western blot

more info or order :

Agrisera product webpage

citations: 1

Reference
Ermakova M, López Calcagno P, Raines C, Furbank R, von Caemmerer S. Overexpression of the Rieske FeS protein of the Cytochrome b6f complex increases C4 photosynthesis in Setaria viridis. Commun Biol. 2019;2:314 pubmed publisher

product information

Product number :

AS10 704

Product name :

PsbA D1 protein of PSII, DE-loop

Immunogen :

KLH-conjugated synthetic peptide, amino acids 234-242 of D1 protein P83755 (AtCg00020)

Host :

Rabbit

Clonality :

Polyclonal

Purity :

Serum

Format :

Lyophilized

Quantity :

50 µl

Tested applications :

Western blot (WB)

Related products :

AS05 084 PsbA antibody, C-terminal rabbit antibodyAS01 016 anti-PsbA C-terminal hen antibodyAS06 124A anti-PsbA, N-terminal rabbit antibodyAS01 016S PsbA protein standard for quantitation and positive controlPlant and algal protein extraction buffer Secondary antibodies

Recommended dilutions :

1 : 10 000, thylakoid fraction (WB)

Expected apparent MW :

38 28-30 kDa

Confirmed reactivity :

Arabidopsis thaliana, Chlamydomonas reinhardii, Hordeum vulgare, Medicago truncatula Neochloris oleoabundans UTEX 1185 (chlorophyta), Physcomitrella patens, Pisum sativum, Silene vulgaris, Sinapsis alba, Spinacia oleracea, Synechococcus sp. PCC 7942, Synechocystis sp. PCC6803

Predicted reactivity :

Cucumis sativus, Glycine max, Nicotiana tabacum, Oryza sativa, Populus balsamifera, Ricinus communis, Zea mays, Vitis vinifera

Not reactive in :

No confirmed exceptions from predicted reactivity are currently known.

Additional information :

Antibody is recognizing a 23 kDa fragment in spinach and Arabidopsis thylakoidsfor usage on total cell extracts the dilution needs to be determined experimentally.

Selected references :

Rantala and Tikkanen et al. (2018). Phosphorylationâ€induced lateral rearrangements of thylakoid protein complexes upon light acclimation. Plant Direct Vol. 2, Issue 2. Wu et al. (2018). Control of Retrograde Signaling by Rapid Turnover of GENOMES UNCOUPLED 1. Plant Physiol. 2018 Jan 24. pii: pp.00009.2018. doi: 10.1104/pp.18.00009. Giovanardi et al. (2017). Higher packing of thylakoid complexes ensures a preserved Photosystem II activity in mixotrophic Neochloris oleoabundans. Algal Research, Volume 25, July 2017, Pages 322-332. Kale et al. (2017). Amino acid oxidation of the D1 and D2 proteins by oxygen radicals during photoinhibition of Photosystem II. Proc Natl Acad Sci U S A. 2017 Mar 14;114(11):2988-2993. doi: 10.1073/pnas.1618922114. Mazur et al. (2016). Overlapping toxic effect of long term thallium exposure on white mustard (Sinapis alba L.) photosynthetic activity. BMC Plant Biol. 2016 Sep 2;16(1):191. doi: 10.1186/s12870-016-0883-4. Kowalewska et al. (2016). Three-dimensional visualization of the internal plastid membrane network during runner bean chloroplast biogenesis. Dynamic model of the tubular-lamellar transformation. The Plant Cell March 21, 2016 tpc.01053.2015. Karlsson et al. (2015). The Arabidopsis thylakoid transporter PHT4;1 influences phosphate availability for ATP synthesis and plant growth. Plant J. 2015 Aug 8. doi: 10.1111/tpj.12962. MalnoÃ« et al. (2014). Thylakoid FtsH Protease Contributes to Photosystem II and Cytochrome b6f Remodeling in Chlamydomonas reinhardtii under Stress Conditions. Plant Cell, Jan 21. Sobrino-Plata et al. (2014). Glutathione is a key antioxidant metabolite to cope with mercury and cadmium stress. Plant Soil, DOI 10.1007/s11104-013-2006-4. Block et al. (2013). Functional Modeling Identifies Paralogous Solanesyl Diphosphate Synthases that Assemble the Side Chain of Plastoquinone-9 in Plastids. J Biol Chem. Aug 2.Spetea et al. (1999). GTP bound to chloroplast thylakoid membranes is required for light-induced, multienzyme degradation of the photosystem II D1 protein. PNAS 96: 6547-6552.

Pubmed ID's :

29367233, 28265052, 27590049, 26255788, 24449688, 23913686, 10339625

Background :

The psbA gene has been cloned from many species of plants, green algae, and cyanobacteria. The psbA gene is located in the chloroplast genome and encodes for the D1 protein, a core component of Photosystem II. PsbA/D1 is rapidly cycled under illumination in all oxygenic photobionts. Tracking PsbA pools using the Global PsbA antibody can show the functional content of Photosystem II in a wide range of samples. Alternative names: 32 kDa thylakoid membrane protein, photosystem II protein D1

Reconstitution :

For reconstitution add 50 µl of sterile water.

Storage :

Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Additional information :

Antibody will detect 23 kDa N-terminal fragment

UniProt number :

P83755

TAIR number :

ATCG00020

more info or order :

Agrisera product webpage